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Title

Genetic-molecular Sources of Multidrug Resistance of Salmonella Isolated from Eggs

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  0-0

Abstract

 Background: Bacteria can be transmitted to consumers around the world due to the movement of animals, and food products. The current study's goal is to look into the genetic and molecular causes of Salmonella isolated from eggs' multiDrug Resistance. Methods: 500 egg samples from 37 Iranian brands were extracted from Salmonella yolk and shell source and after confirmation by differential phenotypic methods and resistance test for Tetracycline, cellophanamide and Nitrofurantoin-furazolidone antibiotics were performed. DNA of strains was extracted and using PCR with specific primers, the presence of tetA, tetB, tetC, tetD, tetG, tetE, tetE, tetH, sul (I), sul (II), sul (III), nfsA, nfsB were examined according to control DNA (ATCC14028). Results: Among the genes of teta, tetb. tetc, tetd, tete, tetf, tetg and teth, the presence of tetc was seen in 81% of the samples and not in 19% of them. Other Tetracycline resistance genes were not found in the samples. Tracing of genes related to sulfonamide resistance by PCR showed that among the Sul1, Sul2, and Sul3 genes, sulfonamide resistance of Sul2 was observed in 95. 5% of the samples. Nitrofurantoin resistance genes in the samples showed that 9. 1% of the samples contained nfsa gene and 4. 5% contained nfsb gene. Other samples did not have any Nitrofurantoin resistance gene. Conclusion: Improper use of antibiotics has caused multiDrug Resistance to Salmonella, so the study of genes and resistance mechanisms in Salmonella strains isolated from produced eggs in Iran is vital.

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    APA: Copy

    Saram, Mohammad, CHAICHI NOSRATI, ARASH, MODIRI, LEILA, & Issazadeh, Khossrow. (2022). Genetic-molecular Sources of Multidrug Resistance of Salmonella Isolated from Eggs. SOCIAL DETERMINANTS OF HEALTH, 8(1), 0-0. SID. https://sid.ir/paper/1126896/en

    Vancouver: Copy

    Saram Mohammad, CHAICHI NOSRATI ARASH, MODIRI LEILA, Issazadeh Khossrow. Genetic-molecular Sources of Multidrug Resistance of Salmonella Isolated from Eggs. SOCIAL DETERMINANTS OF HEALTH[Internet]. 2022;8(1):0-0. Available from: https://sid.ir/paper/1126896/en

    IEEE: Copy

    Mohammad Saram, ARASH CHAICHI NOSRATI, LEILA MODIRI, and Khossrow Issazadeh, “Genetic-molecular Sources of Multidrug Resistance of Salmonella Isolated from Eggs,” SOCIAL DETERMINANTS OF HEALTH, vol. 8, no. 1, pp. 0–0, 2022, [Online]. Available: https://sid.ir/paper/1126896/en

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