Favipiravir Determination in Pharmaceutical Formulation via HPLC Chromatographic Approach

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Aydinoglu Marinelli Sabriye; Bozyel Metin

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF CHEMISTRY AND CHEMICAL ENGINEERING Year:2023 | Volume:42 | Issue:4 Page(s): 1183-1191

Download Full-Text

View:

Download:

Cites:

Information Journal Paper

Title

Favipiravir Determination in Pharmaceutical Formulation via HPLC Chromatographic Approach

Author(s)

Aydinoglu Marinelli Sabriye | Bozyel Metin | Issue Writer Certificate

Keywords

Favipiravir

HPLC

Method validation

Acid Dissociation Constant

Abstract

Favipiravir is a broad-spectrum antiviral drug and it has increasing interest regarding its potential use in Covid-19 therapy. In the present work, a simple, fast, precise Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC) technique was developed for the quantification of favipiravir (FVP) from pharmaceutical formulation. Moreover, the spectral and chromatographic behavior of FVP was investigated. The developed method was performed using an ACE 5 C18 column (250 mm × 4.6 mm, 5 µm), with the mobile phase composition 10 mM phosphate buffer (pH = 2.5): methanol (80:20, v/v) at a flow rate 0.6 mL/min. The method validation parameters, such as linearity, precision, accuracy, and robustness, were determined. The recovery yields for accuracy were between 99.9 and 101.4 % for three concentrations. The linearity range was determined between 0.5 and 100 µg/mL with a regression coefficient (R2) 0.99998. The limit of detection and limit of quantification values were evaluated as 0.02 µg/mL and 0.05 µg/mL, respectively. The precision of the method was evaluated in inter-day and intra-day precision studies with a relative standard deviation of less than 2%. The method's robustness was investigated using the alteration of flow rate, detection wavelength, and mobile phase ratio. Moreover, the effect of the pH and mobile phase ratio on the capacity factors was analyzed and the pKa value of the FVP was determined chromatographically as 5.03 ± 0.02.

Multimedia

No record.

Cites

No record.

References

No record.

Cite

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans