Methyl-cpg-binding Domain Protein 2 Silencing Inhibits Th17 Differentiation of CD4 + T cells Induced by Ovalbumin

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JIANG YAN; Li Linqiao; Pan Qilu; Du Xiaojing; Han Qian; Ling Feixiang; Li Rou; Mai Lin; Huang ianwei; Chu Shuyuan; Ma Libing

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Journal Paper

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Journal: Iranian Journal of Immunology Year:2023 | Volume:20 | Issue:1 Page(s): 45-56

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Title

Methyl-cpg-binding Domain Protein 2 Silencing Inhibits Th17 Differentiation of CD4 + T cells Induced by Ovalbumin

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Keywords

CD4 + T Cell

IL-17

MBD2

Ovalbumin

Th17 Cell

Abstract

Background: Little is known about MBD2’, s epigenetic regulation n the immune pathogenesis of CD4 + T cell differentiation. Objective: This study attempted to explore the mechanism of methyl-cpg-binding domain protein 2 (MBD2) in CD4+T cell differentiation stimulated by environmental allergen Ovalbumin (OVA). Methods: Mononuclear cells were separated from the spleen tissues of male C57BL/6 mice. The OVA interfered with the differentiation of splenic mononuclear cells and CD4+T cells. The CD4+T cells were obtained by magnetic beads and identified by CD4 labeled antibody. CD4+T cells were transfected with lentivirus to silence MBD2 gene. A methylation quantification kit was used to detect 5-mC levels. Results: The purity of CD4+T cells reached 95. 99% after magnetic beads sorting. Treatment with 200 μ, g/mL OVA stimulated the CD4 + T cells differentiation to Th17 Cells and promoted the secretion of IL-17. After being induced, the Th17 Cell ratio increased. 5-Aza inhibited the Th17 Cell differentiation and the IL-17 level in a dosedependent manner. Under the intervention of the Th17 induction and 5-Aza, MBD2 silencing inhibited the differentiation of Th17 Cell, and decreased the IL-17 and 5-mC levels in the cell supernatants. MBD2 silencing reduced the scale of the Th17 Cell and IL-17 levels in the OVA-treated CD4+T cells. Conclusion: MBD2 affected IL-17 and 5-mC levels by mediating the Th17 Cell differentiation in splenic CD4 + T cells that were interfered with 5-Aza. OVA induced Th17 differentiation and increased IL-17 levels, inhibited by MBD2 silencing.

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APA: Copy

JIANG, YAN, Li, Linqiao, Pan, Qilu, Du, Xiaojing, Han, Qian, Ling, Feixiang, Li, Rou, Mai, Lin, Huang, ianwei, Chu, Shuyuan, & Ma, Libing. (2023). Methyl-cpg-binding Domain Protein 2 Silencing Inhibits Th17 Differentiation of CD4 + T cells Induced by Ovalbumin. IRANIAN JOURNAL OF IMMUNOLOGY (IJI), 20(1), 45-56. SID. https://sid.ir/paper/1145439/en

Vancouver: Copy

JIANG YAN, Li Linqiao, Pan Qilu, Du Xiaojing, Han Qian, Ling Feixiang, Li Rou, Mai Lin, Huang ianwei, Chu Shuyuan, Ma Libing. Methyl-cpg-binding Domain Protein 2 Silencing Inhibits Th17 Differentiation of CD4 + T cells Induced by Ovalbumin. IRANIAN JOURNAL OF IMMUNOLOGY (IJI)[Internet]. 2023;20(1):45-56. Available from: https://sid.ir/paper/1145439/en

IEEE: Copy

YAN JIANG, Linqiao Li, Qilu Pan, Xiaojing Du, Qian Han, Feixiang Ling, Rou Li, Lin Mai, ianwei Huang, Shuyuan Chu, and Libing Ma, “Methyl-cpg-binding Domain Protein 2 Silencing Inhibits Th17 Differentiation of CD4 + T cells Induced by Ovalbumin,” IRANIAN JOURNAL OF IMMUNOLOGY (IJI), vol. 20, no. 1, pp. 45–56, 2023, [Online]. Available: https://sid.ir/paper/1145439/en

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