مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

Leukocyte Reduction Filters Are Reliable and Economic Source for Natural Killer Cell Preparation

Pages

  114-128

Abstract

 Background: An issue that hinders researchers’,access to Natural Killer (NK) cells is their low proportion in peripheral blood leukocytes. This issue is currently addressed by methods involving a series of differentiation and expansions that are time-consuming and expensive. Objective: We have investigated whether the used leukocyte reduction filters, a by-product in the blood transfusion practice that currently is considered waste, can be utilized as a source of the NK cells. Methods: Following the blood donation of 46 donors based on the Iranian Blood Transfusion Organization’, s protocols, a sample of peripheral blood of each donor and the leukocyte reduction filter used in their donation procedure have been obtained. The entrapped cells were flushed back from the leukocyte reduction filters. Both groups of samples were analyzed using an automatic hematological analyzer. NK Cell isolation was done by the MACS negative selection method. The samples have been comparatively analyzed utilizing flow cytometry data of NK cells’,subpopulation compositions, viability, degranulation patterns, and cytotoxic capacity against the K562 cell line. Results: Every major leukocyte population was abundant in the samples extracted from the used leukocyte reduction filters. The NK cells extracted from leukocyte reduction filters did not show any statistically meaningful differences (P<0. 5) from peripheral blood samples in terms of subpopulation composition, viability, degranulation potency, and cytotoxic capacity. Conclusion: Used leukocyte reduction filters can be considered an economic, easy to obtain, and robust source of abundant researchgrade NK cells.

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    APA: Copy

    Rezwani, Moosa, Mazarzaei, Abdulbaset, Abbasi Malati, Zahra, & POURFATHOLLAH, ALI AKBAR. (2023). Leukocyte Reduction Filters Are Reliable and Economic Source for Natural Killer Cell Preparation. IRANIAN JOURNAL OF IMMUNOLOGY (IJI), 20(1), 114-128. SID. https://sid.ir/paper/1145446/en

    Vancouver: Copy

    Rezwani Moosa, Mazarzaei Abdulbaset, Abbasi Malati Zahra, POURFATHOLLAH ALI AKBAR. Leukocyte Reduction Filters Are Reliable and Economic Source for Natural Killer Cell Preparation. IRANIAN JOURNAL OF IMMUNOLOGY (IJI)[Internet]. 2023;20(1):114-128. Available from: https://sid.ir/paper/1145446/en

    IEEE: Copy

    Moosa Rezwani, Abdulbaset Mazarzaei, Zahra Abbasi Malati, and ALI AKBAR POURFATHOLLAH, “Leukocyte Reduction Filters Are Reliable and Economic Source for Natural Killer Cell Preparation,” IRANIAN JOURNAL OF IMMUNOLOGY (IJI), vol. 20, no. 1, pp. 114–128, 2023, [Online]. Available: https://sid.ir/paper/1145446/en

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