SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT Pathway

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Gu Wenfei; Qi Guang Tao; CHEN LI

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Immunology Year:2023 | Volume:20 | Issue:3 Page(s): 335-347

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Information Journal Paper

Title

SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT Pathway

Author(s)

Gu Wenfei | Qi Guang Tao | CHEN LI | Issue Writer Certificate

Keywords

JAK/STAT Pathway

Ovarian Cancer

PD-L1 (CD274)

SPIB

Abstract

Background: Spi-B transcription factor (SPIB) is an E-twenty-six (ETS) transcription factor associated with tumor immunity. Objective: To investigate the functions and mechanisms of SPIB in Ovarian Cancer (OC) cells. Methods: Cell proliferation, apoptosis, migration, and invasion were determined using colony formation, EdU, flow cytometry, and transwell assays, respectively. The binding sites of programmed death-ligand 1 (PD-L1) and SPIB were predicted using the JASPAR database and verified using the ChIP and luciferase reporter assays. Results: SPIB knockdown inhibited OC cell proliferation, migration, and invasion, and significantly boosted apoptosis (p<0. 05). SPIB directly enhanced PD-L1 transcription in OVCAR-3 and SKOV3 cells (p<0. 05). Importantly, the JAK/STAT Pathway was markedly inactivated in OC cells upon SPIB knockdown. SPIB knockdown markedly decreased JAK2 and STAT1 phosphorylation in OVCAR-3 and SKOV3 cells (p<0. 05). Conclusion: These data indicate that SPIB knockdown inhibits OC cell progression by downregulating PD-L1 and inactivating the JAK/STAT Pathway.

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APA: Copy

Gu, Wenfei, Qi, Guang Tao, & CHEN, LI. (2023). SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT Pathway. IRANIAN JOURNAL OF IMMUNOLOGY (IJI), 20(3), 335-347. SID. https://sid.ir/paper/1145616/en

Vancouver: Copy

Gu Wenfei, Qi Guang Tao, CHEN LI. SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT Pathway. IRANIAN JOURNAL OF IMMUNOLOGY (IJI)[Internet]. 2023;20(3):335-347. Available from: https://sid.ir/paper/1145616/en

IEEE: Copy

Wenfei Gu, Guang Tao Qi, and LI CHEN, “SPIB Knockdown Inhibits the Immune Escape of Ovarian Cancer Cells by Reducing PD-L1 (CD274) Expression and Inactivating the JAK/STAT Pathway,” IRANIAN JOURNAL OF IMMUNOLOGY (IJI), vol. 20, no. 3, pp. 335–347, 2023, [Online]. Available: https://sid.ir/paper/1145616/en

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