Investigating the frequency of SHV gene encoding ESBL in E.coli strains isolated from urine samples of patients who were referred to hospitals and medical centers in Semnan city

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Shabani Alireza; Shabani Ali Akbar; Nasr Reza; Ardakanian Maryam; Ravai Sima; Afghan Pertabi Alireza; Anani Hossein; Shabani Hoda; Abu Zari Ali; Qurbani Majid; Mehrjo Fateme

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Journal Paper

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Journal: Koomesh Year:2023 | Volume:25 | Issue:6 Page(s): 659-667

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Information Journal Paper

Title

Investigating the frequency of SHV gene encoding ESBL in E.coli strains isolated from urine samples of patients who were referred to hospitals and medical centers in Semnan city

Author(s)

Keywords

ESBLQ4

Escherichia coliQ4

Urinary Tract InfectionQ4

SHVQ4

Abstract

2Introduction: Numerous reports indicate the spread of multiple drug resistances through different types of Extended Spectrum Beta Lactamase (ESBL), including enzymes resulting from SHV gene expression in different parts of the world, which is one of the major medical and therapeutic problems. Nowadays, investigating the role of Escherichia coli bacteria in various infections, including hospital infections, and the amount of use of different antibiotics in treatment, considering the increasing resistance of bacteria causing infection to antibiotics, is a necessity. The purpose of this research project was to investigate the prevalence of the SHV gene as one of the genes encoding ESBL in infectious bacteria including E. coli strains. Materials and Methods: Sampling and isolation of Escherichia coli collected from clients suspected of Urinary Tract Infection using standard methods and antibiogram test using disc-diffusion method were performed on them. To identify strains producing broad-spectrum beta-lactamases (ESBLs), nitrocephene-resistant isolates rechecked with the combined disc method to definitively detect the production of broad-spectrum beta-lactamase enzymes (ESBLs) with the use of pairs of ceftazidime, cefotaxime, cefotaxime, and ceftazidime antibiotic discs with and without clavulanic acid purchased from British Mast Company were tested. By extracting DNA from them using specific primers designed, evaluated, and prepared for the SHV gene, and performing PCR, the presence or absence of the SHV gene in the above strains was evaluated. Results: E. coli strains were isolated from 151 urinary samples (37.75)%. Isolates resistant to nitrocephene were considered as possible strains producing extended-spectrum beta-lactamases (ESBLs), the result of the confirmatory phenotypic test on probable positive (+) ESBL strains, in 33 cases (67.47) % of them were positive. By performing PCR using a pair of specific primers designed and prepared to detect and identify the SHV gene, the result of this test was also positive in 9 cases (72.72) % of them. Conclusion: Using molecular methods along with phenotypic methods to accurately diagnose infectious agents, even their VBNC (viable but non-culturable) forms, and resistance genes can make the effectiveness of "molecular epidemiology" methods in tracking and increasing the fight against infections, including hospital infections.

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