CHOOSING THE BEST VIRAL VECTOR FOR SPECIFIC KNOCKING DOWN OF CREB1 GENE IN 293T CELLS

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ESMAEILZADEH ELIKA; BANAN MEHDI

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Journal: GENETICS IN THE 3RD MILLENNIUM Year:2011 | Volume:9 | Issue:1 Page(s): 2276-2279

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Information Journal Paper

Title

CHOOSING THE BEST VIRAL VECTOR FOR SPECIFIC KNOCKING DOWN OF CREB1 GENE IN 293T CELLS

Author(s)

ESMAEILZADEH ELIKA | BANAN MEHDI | Issue Writer Certificate

Keywords

RNAQ2

SMALL INTERFERINGQ2

CREB1Q2

QRT-PCRQ2

Abstract

In this study, several designed CREB1-specific shRNAs cloned into lentiviral transfer vectors were screened in293T by transient transfection assays. Relative CREB1 expression was determined by relative QRT-PCR. All of the designed shRNAs gave some level of knockdown. One shRNA was more effective for knocking down the specific gene among others. The predicted siRNAs activities as determined by Reynolds et al were compared to the actual potencies. Our results showed a general correlation between the predicted and actual siRNA activities.

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APA: Copy

ESMAEILZADEH, ELIKA, & BANAN, MEHDI. (2011). CHOOSING THE BEST VIRAL VECTOR FOR SPECIFIC KNOCKING DOWN OF CREB1 GENE IN 293T CELLS. GENETICS IN THE 3RD MILLENNIUM, 9(1), 2276-2279. SID. https://sid.ir/paper/117199/en

Vancouver: Copy

ESMAEILZADEH ELIKA, BANAN MEHDI. CHOOSING THE BEST VIRAL VECTOR FOR SPECIFIC KNOCKING DOWN OF CREB1 GENE IN 293T CELLS. GENETICS IN THE 3RD MILLENNIUM[Internet]. 2011;9(1):2276-2279. Available from: https://sid.ir/paper/117199/en

IEEE: Copy

ELIKA ESMAEILZADEH, and MEHDI BANAN, “CHOOSING THE BEST VIRAL VECTOR FOR SPECIFIC KNOCKING DOWN OF CREB1 GENE IN 293T CELLS,” GENETICS IN THE 3RD MILLENNIUM, vol. 9, no. 1, pp. 2276–2279, 2011, [Online]. Available: https://sid.ir/paper/117199/en

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