EXTRACTION AND PURIFICATION OF GLYCOMACROPEPTIDE FROM WHEY TO BE USED AS A SOURCE OF PROTEIN FOR PHENYLKETONURIA PATIENTS

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JAVANMARD M.; LIGHVANI H.; GHIASI TARZI B.; RASHIDI A.

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Journal: JOURNAL OF NUTRITION SCIENCES & FOOD TECHNOLOGY Year:2012 | Volume:7 | Issue:2 (25) Page(s): 9-16

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Title

EXTRACTION AND PURIFICATION OF GLYCOMACROPEPTIDE FROM WHEY TO BE USED AS A SOURCE OF PROTEIN FOR PHENYLKETONURIA PATIENTS

Author(s)

JAVANMARD M. | LIGHVANI H. | GHIASI TARZI B. | RASHIDI A. | Issue Writer Certificate

Keywords

EXTRACTIONQ2

GLYCOMACROPEPTIDE (GMP)Q3

PHENYLKETONURIAQ3

PURIFICATIONQ2

WHEYQ3

Abstract

Background and Objective: PHENYLKETONURIA (PKU) is a genetic disease. Patients suffering from PKU must adhere to a lifelong low-phenylalanine diet. Glycomacropeptide (GMP) is a unique source of protein for PKU patients, because it contains no phenylalanine. The objective of this study was to extract GMP from WHEY and purify it in order to obtain a product with a minimum phenylalanine content. The ultrafiltration membrane technology was used to separate GMP from the WHEY protein concentrate solution at different pH values.Materials and Methods: Two ultrafilteration disc membranes with 50 and 10 kDa cut-off were used to extract glycomacropeptide from a solution of WHEY protein concentrtae (10% protein w/v).The experiments in triplicates were performed at the ambient temperature (25±2 °C) and a pH of 3.5, 4 or 4.5.The diafilteration technique was used for PURIFICATION of glycomacropeptide in both ultrafilteration phases, the protein, phenylalanine, and non-protein nitrogen (NPN) contents were measured.Results: The phenylalanine and NPN contents of the ultrafiltered WHEY at a pH of 4 were at the lowest and highest level, respectively, indicating the high purity and recovery rate of glycomacropeptide.Conclusion: Sweet WHEY, the major type of WHEY produced in the country, is the best alternative available for producing a new food source for PHENYLKETONURIA patients.

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APA: Copy

JAVANMARD, M., LIGHVANI, H., GHIASI TARZI, B., & RASHIDI, A.. (2012). EXTRACTION AND PURIFICATION OF GLYCOMACROPEPTIDE FROM WHEY TO BE USED AS A SOURCE OF PROTEIN FOR PHENYLKETONURIA PATIENTS. JOURNAL OF NUTRITION SCIENCES & FOOD TECHNOLOGY, 7(2 (25)), 9-16. SID. https://sid.ir/paper/121512/en

Vancouver: Copy

JAVANMARD M., LIGHVANI H., GHIASI TARZI B., RASHIDI A.. EXTRACTION AND PURIFICATION OF GLYCOMACROPEPTIDE FROM WHEY TO BE USED AS A SOURCE OF PROTEIN FOR PHENYLKETONURIA PATIENTS. JOURNAL OF NUTRITION SCIENCES & FOOD TECHNOLOGY[Internet]. 2012;7(2 (25)):9-16. Available from: https://sid.ir/paper/121512/en

IEEE: Copy

M. JAVANMARD, H. LIGHVANI, B. GHIASI TARZI, and A. RASHIDI, “EXTRACTION AND PURIFICATION OF GLYCOMACROPEPTIDE FROM WHEY TO BE USED AS A SOURCE OF PROTEIN FOR PHENYLKETONURIA PATIENTS,” JOURNAL OF NUTRITION SCIENCES & FOOD TECHNOLOGY, vol. 7, no. 2 (25), pp. 9–16, 2012, [Online]. Available: https://sid.ir/paper/121512/en

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