EXPRESSION OF WNT AND NOTCH SIGNALING PATHWAYS ARE INCREASED IN CANCER STEM-LIKE CELLS IN METASTATIC MELANOMA CELL LINE (A375)

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MEHDIPOUR MARYAM; KEIGHOBADI FAEZEH; FIROUZI JAVAD; KHOSRAVANI PARDIS; EBRAHIMI MARZIEH

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Jundishapur Scientific Medical Journal Year:2017 | Volume:16 | Issue:6 Page(s): 643-652

Download Full-Text

Persian Verion

View:

2,020

Download:

Cites:

Information Journal Paper

Title

EXPRESSION OF WNT AND NOTCH SIGNALING PATHWAYS ARE INCREASED IN CANCER STEM-LIKE CELLS IN METASTATIC MELANOMA CELL LINE (A375)

Author(s)

Keywords

MELANOMAQ2

CANCER STEM CELLQ2

SPHEREQ1

NOTCHQ1

WNTQ1

Abstract

Backgound and Objective: CANCER STEM CELLs assume to be responsible for increasing cancer properties such as migration, and drug sensitivity. A lot of studies revealed signaling pathways are enhanced in CANCER STEM CELLs. MELANOMA is well-known as heterogeneous cancer and its severity in treatment. These can be attributed to the existence of CANCER STEM CELLs. In this study, we first aimed to separate CANCER STEM CELLs (cancer stem-like cells) by SPHERE formation, characterized them, and second examine the expression of WNT and NOTCH signaling pathway genes related to adherent cells. Subjects and Methods: Adherent cell were cultured in the non-adherent condition with serum-free media; SPHEREs obtained, then SPHERE formation, clonogenic assay, expression of CD133 and Nestin proteins, Nanog, NESTIN and Oct4 genes as stem related genes were assessed in comparison to adherent cells. In addition, NOTCH and WNT signaling pathways genes in both adherent and SPHEREs cells evaluated. Results: SPHERE formation, clonogenic capacity, expression of Nestin protein, but not CD133 were increased in SPHERE cells in comparession to adherent cells. They also overexpressed β-catenin, cyclinD1, and c-Myc as WNT down-stream genes, NOTCH1, HES1 as NOTCH down-stream genes, Nanog, and NESTIN as stem-related genes. Conclusion: These results suggest that SPHERE culture model could be a proper experimental method to separate cancer stem-like cells. Our data also support two important pathways are overactivated in MELANOMA cancer stem-like cells which must be considered in targeting therapy.

Cites

No record.

References

No record.

Cite

APA: Copy

MEHDIPOUR, MARYAM, KEIGHOBADI, FAEZEH, FIROUZI, JAVAD, KHOSRAVANI, PARDIS, & EBRAHIMI, MARZIEH. (2017). EXPRESSION OF WNT AND NOTCH SIGNALING PATHWAYS ARE INCREASED IN CANCER STEM-LIKE CELLS IN METASTATIC MELANOMA CELL LINE (A375). JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL, 16(6 ), 643-652. SID. https://sid.ir/paper/12481/en

Vancouver: Copy

MEHDIPOUR MARYAM, KEIGHOBADI FAEZEH, FIROUZI JAVAD, KHOSRAVANI PARDIS, EBRAHIMI MARZIEH. EXPRESSION OF WNT AND NOTCH SIGNALING PATHWAYS ARE INCREASED IN CANCER STEM-LIKE CELLS IN METASTATIC MELANOMA CELL LINE (A375). JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL[Internet]. 2017;16(6 ):643-652. Available from: https://sid.ir/paper/12481/en

IEEE: Copy

MARYAM MEHDIPOUR, FAEZEH KEIGHOBADI, JAVAD FIROUZI, PARDIS KHOSRAVANI, and MARZIEH EBRAHIMI, “EXPRESSION OF WNT AND NOTCH SIGNALING PATHWAYS ARE INCREASED IN CANCER STEM-LIKE CELLS IN METASTATIC MELANOMA CELL LINE (A375),” JUNDISHAPUR SCIENTIFIC MEDICAL JOURNAL, vol. 16, no. 6 , pp. 643–652, 2017, [Online]. Available: https://sid.ir/paper/12481/en

Related Journal Papers