IN VITRO COMPARISON OF ANTIMICROBIAL EFFECTS OF COLCHICINE AND CALCIUM HYDROXIDE ON SALIVARY NORMAL FLORA AND FIVE MICROBIAL STRAINS

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BAREKATEIN BEHNAZ; FARHAD ALI REZA; REFAEI POORANDOKHT

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Title

IN VITRO COMPARISON OF ANTIMICROBIAL EFFECTS OF COLCHICINE AND CALCIUM HYDROXIDE ON SALIVARY NORMAL FLORA AND FIVE MICROBIAL STRAINS

Author(s)

BAREKATEIN BEHNAZ | FARHAD ALI REZA | REFAEI POORANDOKHT | Issue Writer Certificate

Keywords

ANTIBACTERIALQ3

CALCIUM HYDROXIDEQ2

COLCHICINEQ2

MINIMUM BACTERICIDAL CONCENTRATIONQ2

MINIMUM INHIBITORY CONCENTRATIONQ2

Abstract

Introduction: Resistant microbial strains are major factors involved in root canal treatment failure.The aim of this study was to compare the antimicrobial properties of different concentrations of COLCHICINE and CALCIUM HYDROXIDE.Materials and Methods: In this in vitro study, suspensions containing 5x105 colony-forming units of E. coli, E. faecalis, P. aeuroginosa, S. aureus, normal salivary flora and C. albicans and different concentrations (1, 0.5, 0.25, 0.125 and 0.0625 mg/mL) of CALCIUM HYDROXIDE and COLCHICINE were prepared. The samples were incubated at 37oC for 24 hours. Then turbidity of the samples was evaluated to determine MINIMUM INHIBITORY CONCENTRATION (MIC). Then 0.1 mL of the broth with no turbidity was cultured on solid medium to determine MINIMUM BACTERICIDAL CONCENTRATION (MBC). In order to make sure of the accuracy of the process, positive, negative and standard controls were also prepared.Results: In evaluation of sample turbidity after 24 hours, all the tubes containing CALCIUM HYDROXIDE and positive controls showed complete turbidity. In tubes containing COLCHICINE turbidity was found in all the bacterial categories. Only tubes containing Candida albicans with concentrations of 1, 0.5 and 0.25 mg/mL of COLCHICINE were clear and lower concentrations showed turbidity. In evaluation of formation of colonies on Sab. Dextrose agar, only plates with 1 mg/mL of COLCHICINE showed no growth.Conclusion: Only concentrations of 0.5 and 0.25 mg/mL of COLCHICINE exhibited inhibitory effects and 1 mg/mL exhibited lethal effect on Candida albicans. None of the two drugs exhibited any inhibitory or lethal effects on other microbial strains.

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APA: Copy

BAREKATEIN, BEHNAZ, FARHAD, ALI REZA, & REFAEI, POORANDOKHT. (2012). IN VITRO COMPARISON OF ANTIMICROBIAL EFFECTS OF COLCHICINE AND CALCIUM HYDROXIDE ON SALIVARY NORMAL FLORA AND FIVE MICROBIAL STRAINS. JOURNAL OF ISFAHAN DENTAL SCHOOL, 7(4), 409-417. SID. https://sid.ir/paper/126164/en

Vancouver: Copy

BAREKATEIN BEHNAZ, FARHAD ALI REZA, REFAEI POORANDOKHT. IN VITRO COMPARISON OF ANTIMICROBIAL EFFECTS OF COLCHICINE AND CALCIUM HYDROXIDE ON SALIVARY NORMAL FLORA AND FIVE MICROBIAL STRAINS. JOURNAL OF ISFAHAN DENTAL SCHOOL[Internet]. 2012;7(4):409-417. Available from: https://sid.ir/paper/126164/en

IEEE: Copy

BEHNAZ BAREKATEIN, ALI REZA FARHAD, and POORANDOKHT REFAEI, “IN VITRO COMPARISON OF ANTIMICROBIAL EFFECTS OF COLCHICINE AND CALCIUM HYDROXIDE ON SALIVARY NORMAL FLORA AND FIVE MICROBIAL STRAINS,” JOURNAL OF ISFAHAN DENTAL SCHOOL, vol. 7, no. 4, pp. 409–417, 2012, [Online]. Available: https://sid.ir/paper/126164/en

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