مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Title

IDENTIFICATION OF ENTEROCOCCI FAECALIS AND E. FAEACIUM PATHOGENS VIA TEHRAN HOSPITALS CLINICAL SAMPLES BY PHENOTYPIC AND GENOTYPIC METHODS AND EVALUATION OF ANTIMICROBIAL SUSCEPTIBILITY IN 2015

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  1-8

Abstract

 Introduction: Epidemiology of Enterococcus infections has attracted much attention. Precise identification of pathogenic strains can be effective in the control process of microorganism ANTIBIOTIC RESISTANCE. This study aimed to identify the Enterococcus species isolated from hospitals in Tehran and examine their ANTIBIOTIC RESISTANCE pattern by phenotypic and genotypic methods.Methods & Materials: This study was performed on 400 clinical samples from different hospitals in Tehran during 2015-2016. Specific cultures and biochemical tests were used to identify Enterococcus, distinguish E.faecalis and E. faeacium species and PCR method was used to identify Enterococcus species.Antibiotic susceptibility was examined using Kirby-Bauer disc diffusion, and CLSI and vancomycin MIC were measured using broth dilution.Results: Of the 400 samples, 278 Enterococcus species were recognized. Phenotypic methods recognized 70.86% E. faecalis, 15.46% E. faeacium and 13.68% other species. PCR identified 72.3% E. faecalis, 10.43% E. faeacium and 17.27% other Enterococcus species. Results of the antibiograms showed the highest resistance (83.34%) to quinupristin/dalfopristin, and the lowest (1.41%) to linezolid. Also, resistance to vancomycin was observed in 5.95% with MIC³ 512 mg/ml in 9 cases.Conclusion: Rapid diagnosis can prevent massive outbreaks of Enterococcus. Given the prevalence of vancomycin resistance in Enterococcus, preventive measures are imperative. The right antibiotics should be prescribed according to the resistance patterns after susceptibility test is performed for each patient.

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    APA: Copy

    MASOUMI ZAVARYANI, SARA, MIRNEJAD, REZA, ZARE KARIZI, SHOHREH, PIRANFAR, VAHHAB, & BAGHERI BEJESTANI, OZRA. (2016). IDENTIFICATION OF ENTEROCOCCI FAECALIS AND E. FAEACIUM PATHOGENS VIA TEHRAN HOSPITALS CLINICAL SAMPLES BY PHENOTYPIC AND GENOTYPIC METHODS AND EVALUATION OF ANTIMICROBIAL SUSCEPTIBILITY IN 2015. PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL), 14(3 ), 1-8. SID. https://sid.ir/paper/130847/en

    Vancouver: Copy

    MASOUMI ZAVARYANI SARA, MIRNEJAD REZA, ZARE KARIZI SHOHREH, PIRANFAR VAHHAB, BAGHERI BEJESTANI OZRA. IDENTIFICATION OF ENTEROCOCCI FAECALIS AND E. FAEACIUM PATHOGENS VIA TEHRAN HOSPITALS CLINICAL SAMPLES BY PHENOTYPIC AND GENOTYPIC METHODS AND EVALUATION OF ANTIMICROBIAL SUSCEPTIBILITY IN 2015. PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL)[Internet]. 2016;14(3 ):1-8. Available from: https://sid.ir/paper/130847/en

    IEEE: Copy

    SARA MASOUMI ZAVARYANI, REZA MIRNEJAD, SHOHREH ZARE KARIZI, VAHHAB PIRANFAR, and OZRA BAGHERI BEJESTANI, “IDENTIFICATION OF ENTEROCOCCI FAECALIS AND E. FAEACIUM PATHOGENS VIA TEHRAN HOSPITALS CLINICAL SAMPLES BY PHENOTYPIC AND GENOTYPIC METHODS AND EVALUATION OF ANTIMICROBIAL SUSCEPTIBILITY IN 2015,” PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL), vol. 14, no. 3 , pp. 1–8, 2016, [Online]. Available: https://sid.ir/paper/130847/en

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