EVALUATING THE ABILITY OF BIOFILM FORMATION IN ESCHERICHIA COLI ISOLATED FROM CLINICAL SAMPLES IN ZAHEDAN

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JALILIAN SARA; ROKHBAKHSH ZAMIN FAROKH

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Journal: Pars Journal of Medical Sciences Year:2017 | Volume:15 | Issue:1 Page(s): 36-42

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Information Journal Paper

Title

EVALUATING THE ABILITY OF BIOFILM FORMATION IN ESCHERICHIA COLI ISOLATED FROM CLINICAL SAMPLES IN ZAHEDAN

Author(s)

JALILIAN SARA | ROKHBAKHSH ZAMIN FAROKH | Issue Writer Certificate

Keywords

ESCHERICHIA COLIQ2

ANTIBIOTIC RESISTANCEQ2

PCRQ2

BIOFILMQ2

Abstract

Introduction: ESCHERICHIA COLI (E.coli) is the main causative pathogen in urinary tract infections (UTIs). ANTIBIOTIC RESISTANCE based on Extended Spectrum -Lactamase (ESBL) producing ESCHERICHIA COLI strains is reported to be the cause of community and hospital acquired infections therefore this this is an important problem in public health. The aim of this study was to identify the blaTEM, blaSHV and CTX genes in clinical isolates of E. coli recovered from patients with UTIs in Zahedan, Iran.Methods and Materials: ESCHERICHIA COLI isolates (N=60) were analyzed by biochemistry and phenotypic methods. Each of the initial E. coli screening test isolate was investigated for the presence of blaTEM, blaSHV and blaCTX-M genes via polymerase chain reaction (PCR) using gene-specific primers.Results: Sixty tested isolates; the prevalence of blaTEM and blaCTX-M genes was determined to be 68.3%, 46.6% but blaSHV gene was not detected. Based on phenotypic tests, BIOFILM capacity was detected in 10 clinical isolates.Conclusions: The results of this study show that the use of adequate doses of antibiotics in the treatment of infections may be caused by BIOFILMs are emphasized. Rapid detection of BIOFILM formation, therefore isolates have the ability to decide care and management is necessary.

Cites

Evaluation of pap and sfa Genes Relative Frequency P and S Fimbriae Encoding of Uropathogenic Escherichia coli Isolated from Hospitals and Medical Laboratories; Yasuj City, Southwest Iran

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APA: Copy

JALILIAN, SARA, & ROKHBAKHSH ZAMIN, FAROKH. (2017). EVALUATING THE ABILITY OF BIOFILM FORMATION IN ESCHERICHIA COLI ISOLATED FROM CLINICAL SAMPLES IN ZAHEDAN. PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL), 15(1 ), 36-42. SID. https://sid.ir/paper/130931/en

Vancouver: Copy

JALILIAN SARA, ROKHBAKHSH ZAMIN FAROKH. EVALUATING THE ABILITY OF BIOFILM FORMATION IN ESCHERICHIA COLI ISOLATED FROM CLINICAL SAMPLES IN ZAHEDAN. PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL)[Internet]. 2017;15(1 ):36-42. Available from: https://sid.ir/paper/130931/en

IEEE: Copy

SARA JALILIAN, and FAROKH ROKHBAKHSH ZAMIN, “EVALUATING THE ABILITY OF BIOFILM FORMATION IN ESCHERICHIA COLI ISOLATED FROM CLINICAL SAMPLES IN ZAHEDAN,” PARS JOURNAL OF MEDICAL SCIENCES (JAHROM MEDICAL JOURNAL), vol. 15, no. 1 , pp. 36–42, 2017, [Online]. Available: https://sid.ir/paper/130931/en

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