IDENTIFICATION OF BLACTX-M, BLASHV, AND BLATEM GENES IN PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM HUMAN AND ANIMAL SAMPLES USING MULTIPLEX-PCR METHOD

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FARZALI SHIREHJINI FARZANEH; AMINI KIOUMARS; FATAHI HOSSEIN

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Journal: QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL Year:2017 | Volume:10 | Issue:11 Page(s): 51-60

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Title

IDENTIFICATION OF BLACTX-M, BLASHV, AND BLATEM GENES IN PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM HUMAN AND ANIMAL SAMPLES USING MULTIPLEX-PCR METHOD

Author(s)

FARZALI SHIREHJINI FARZANEH | AMINI KIOUMARS | FATAHI HOSSEIN | Issue Writer Certificate

Keywords

PSEUDOMONAS AERUGINOSAQ2

BETAQ2

LACTAMASEQ1

ANTIBIOTIC SUSCEPTIBILITYQ1

DRUG HYPERSENSITIVITYQ1

Abstract

Background and Objectives: PSEUDOMONAS AERUGINOSA is one of the most important causes of opportunistic infections. The infection caused by P. aeruginosa is commonly severe and life-threatening and hard to treat due to limited susceptibility to antibiotics and development of resistance during treatment. The aim of the present study was to identify blaCTX-M, blaSHV, and blaTEM genes in P. ‎aeruginosa strains isolated from human and animal samples using Multiplex-PCR method.‎Methods: In this descriptive cross-sectional study, a total of 120 isolates of P. aeruginosa were obtained from of human samples and animal raw milk. ANTIBIOTIC SUSCEPTIBILITY test was determined by gel diffusion method and according to CLSI guidelines. Cellular DNA was extracted by CinnaPure-DNA (Cell culture, Tissues, Gram-negative Bacteria and CSF) and Multiplex-PCR was performed for identification of blaCTX-M, blaSHV, and blaTEM genes.Results: In this study, the highest resistance in human and animal samples, was seen to amoxicillin and amikacin (100%) ‎and the lowest resistance was to ciprofloxacin with frequency of 90% in human ‎samples and 93.4% in animal samples. Based on the CDT results, 37 (60.8%) isolates showed ESBLs phenotype, and based on the M-PCR results, 26 ‎‎ (21.6%) and 41 (34.2%) strains had blaCTX-M and blaTEM genes, respectively.Conclusion: The results of this study indicated that P. aeruginosa strains have high levels of resistance. Also, the frequency of these genes is high among human and animal isolates, therefore controlling these bacteria in humans and animals is of particular importance.

Cites

Prevalence of SHV, TEM, CTX-M and OXA-48 β-Lactamase Genes in Clinical Isolates of Pseudomonas aeruginosa in Bandar-Abbas, Iran

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APA: Copy

FARZALI SHIREHJINI, FARZANEH, AMINI, KIOUMARS, & FATAHI, HOSSEIN. (2017). IDENTIFICATION OF BLA_CTX-M, BLA_SHV, AND BLA_TEM GENES IN PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM HUMAN AND ANIMAL SAMPLES USING MULTIPLEX-PCR METHOD. QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, 10(11), 51-60. SID. https://sid.ir/paper/132013/en

Vancouver: Copy

FARZALI SHIREHJINI FARZANEH, AMINI KIOUMARS, FATAHI HOSSEIN. IDENTIFICATION OF BLA_CTX-M, BLA_SHV, AND BLA_TEM GENES IN PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM HUMAN AND ANIMAL SAMPLES USING MULTIPLEX-PCR METHOD. QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL[Internet]. 2017;10(11):51-60. Available from: https://sid.ir/paper/132013/en

IEEE: Copy

FARZANEH FARZALI SHIREHJINI, KIOUMARS AMINI, and HOSSEIN FATAHI, “IDENTIFICATION OF BLA_CTX-M, BLA_SHV, AND BLA_TEM GENES IN PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM HUMAN AND ANIMAL SAMPLES USING MULTIPLEX-PCR METHOD,” QOM UNIVERSITY OF MEDICAL SCIENCES JOURNAL, vol. 10, no. 11, pp. 51–60, 2017, [Online]. Available: https://sid.ir/paper/132013/en

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