EVALUATION OF PCR METHOD FOR DIAGNOSIS OF GROUP B STREPTOCOCCUS CARRIAGE IN PREGNANT WOMEN

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BAKHTIARI ROUNAK; SOLTAN DALAL MOHAMMAD MAHDI; ZAEIMI YAZDI J.; FALAH JALIL; AMIR MOZAFARI N.; POURMAND M.R.; HAJIKHANI S.

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Journal: Iranian Journal of Medical Microbiology Year:2007 | Volume:1 | Issue:2 Page(s): 1-8

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Title

EVALUATION OF PCR METHOD FOR DIAGNOSIS OF GROUP B STREPTOCOCCUS CARRIAGE IN PREGNANT WOMEN

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Keywords

GROUP B STREPTOCOCCUSQ4

PREGNANT WOMENQ4

PCR ASSAYQ4

Abstract

Background and Objectives: GROUP B STREPTOCOCCUS (GBS) (Streptococcus agalactiae) is the leading cause of morbidity and mortality of newborn infants and accounted as a leading factor causing septicemia after birth in mothers. Infections in infants are usually acquired by contact with the genital tract of the mothers during labor and delivery. In two last decades, significant progress toward detection, prevention and treatment of PREGNANT WOMEN carrying GBS has been achieved. A rapid screening test for GBS that could accurately identify PREGNANT WOMEN carrying the bacteria at the time of delivery would obviate the need for prenatal screening.The standard method for the diagnosis of GBS colonization consists of culturing vaginal and anal secretions in a selective broth medium which inhibits the growth of other microorganisms. Today, it is accepted that PCR has a high sensitivity and specifically in diagnosis. The goal of this study was to screen pregnant woman carrying GBS by pcg.Material and Methods: Samples were taken from anal and vaginal mucus of 125 PREGNANT WOMEN who were at 28-38 weeks of ingestion by swab. Samples were tested by standard culture using Todd Hewitt Broth and Blood Agar and also by PCR using primers specific for cjb gene.Results: Culture identified 10 (8%) women as carriage of GBS out of 125 women tested. On the other hand, the PCR ASSAY could identify 12 (9/6%) women positive for GBS. In comparison to culture results, sensitivity, NPV, specificity, and PPV of PCR were 100%, 100%, 98%, and 83%, respectively. The time required for PCR ASSAY and culture were 2h and 36h, respectively.Conclusion: We found that GBS can be detected rapidly and reliably by a PCR ASSAY using combined vaginal and anal secretions from PREGNANT WOMEN at the time of delivery. Also this study shows that the rate of incidence of GBS is high in Iranian PREGNANT WOMEN. We, therefore, recommend screening of PREGNANT WOMEN for detecting of GBS emphatically.

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APA: Copy

BAKHTIARI, ROUNAK, SOLTAN DALAL, MOHAMMAD MAHDI, ZAEIMI YAZDI, J., FALAH, JALIL, AMIR MOZAFARI, N., POURMAND, M.R., & HAJIKHANI, S.. (2007). EVALUATION OF PCR METHOD FOR DIAGNOSIS OF GROUP B STREPTOCOCCUS CARRIAGE IN PREGNANT WOMEN. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 1(2), 1-8. SID. https://sid.ir/paper/132556/en

Vancouver: Copy

BAKHTIARI ROUNAK, SOLTAN DALAL MOHAMMAD MAHDI, ZAEIMI YAZDI J., FALAH JALIL, AMIR MOZAFARI N., POURMAND M.R., HAJIKHANI S.. EVALUATION OF PCR METHOD FOR DIAGNOSIS OF GROUP B STREPTOCOCCUS CARRIAGE IN PREGNANT WOMEN. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2007;1(2):1-8. Available from: https://sid.ir/paper/132556/en

IEEE: Copy

ROUNAK BAKHTIARI, MOHAMMAD MAHDI SOLTAN DALAL, J. ZAEIMI YAZDI, JALIL FALAH, N. AMIR MOZAFARI, M.R. POURMAND, and S. HAJIKHANI, “EVALUATION OF PCR METHOD FOR DIAGNOSIS OF GROUP B STREPTOCOCCUS CARRIAGE IN PREGNANT WOMEN,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 1, no. 2, pp. 1–8, 2007, [Online]. Available: https://sid.ir/paper/132556/en

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