DESIGN AND CONSTRUCTION A RECOMBINANT BCG CONTAINING CYTOSINE DEAMINASE SUICIDE GENE TO INCREASE THE POTENCY OF INTRAVESICAL BCG IN TREATMENT OF BLADDER CANCER

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FEYZ BARAZANDEH A.; KHANAHMAD H.; EBRAHIMIPOUR GH.H.; ABOU ALHASANI M.; MOVASAGH H.A.D.; SOHRABI T.

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Journal: IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY Year:2009 | Volume:2 | Issue:3-4 Page(s): 1-8

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Title

DESIGN AND CONSTRUCTION A RECOMBINANT BCG CONTAINING CYTOSINE DEAMINASE SUICIDE GENE TO INCREASE THE POTENCY OF INTRAVESICAL BCG IN TREATMENT OF BLADDER CANCER

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Keywords

BCGQ4

BLADDER CANCERQ4

IMMUNOTHERAPYQ4

GENE THERAPYQ3

ELECTROPORATIONQ4

Abstract

Background and Objectives: BLADDER CANCER is the second common genitourinary tract cancer in the world. IMMUNOTHERAPY with intravesical Mycobacterium bovis BCG is the selective treatment for superficial BLADDER CANCER. CD/5FC Enzyme-Prodrug system of GENE THERAPY is an alternative technique for controlling BLADDER CANCER. This research is aimed at the design and construction of mycobacterial shuttle vector containing hsp60 promoter, alpha antigen signal sequence and cytosine deaminase (CD) and consequent transformation to M.bovis BCG by ELECTROPORATION. It is assumed that converting of 5-FC to 5-FU via CD will kill more tumor cells and increase the efficacy of this therapeutic method.Material and Method: After amplification of mycobacterial shuttle vector gene fragments containing hsp60 promoter, alpha antigen signal sequence and Saccharomyces cereviseae CD gene by PCR and enzymatic digestion, they were cloned and subcloned in pBGGT and pVN2 respectively. The final plasmid was transformed to M. bovis BCG by ELECTROPORATION method. Results: Sequencing results confirmed the integrity and correctness of final vector which called pHARA. The cells which received pHARA, were cultured on Middlebrook 7H10 agar with 20mg/ml final concentration of kanamycin, and grown during 17 days.Conclusion: Mentioned recombinant BCG (rBCG) secreting CD enzyme in addition to adjuvant property and stimulation of local immunity responses against tumor cells, can also destroy more tumor cells through converting 5-FC to 5-FU via CD enzyme that intensifies BCG efficacy in treatment of superficial BLADDER CANCER.

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APA: Copy

FEYZ BARAZANDEH, A., KHANAHMAD, H., EBRAHIMIPOUR, GH.H., ABOU ALHASANI, M., MOVASAGH, H.A.D., & SOHRABI, T.. (2009). DESIGN AND CONSTRUCTION A RECOMBINANT BCG CONTAINING CYTOSINE DEAMINASE SUICIDE GENE TO INCREASE THE POTENCY OF INTRAVESICAL BCG IN TREATMENT OF BLADDER CANCER. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 2(3-4), 1-8. SID. https://sid.ir/paper/132594/en

Vancouver: Copy

FEYZ BARAZANDEH A., KHANAHMAD H., EBRAHIMIPOUR GH.H., ABOU ALHASANI M., MOVASAGH H.A.D., SOHRABI T.. DESIGN AND CONSTRUCTION A RECOMBINANT BCG CONTAINING CYTOSINE DEAMINASE SUICIDE GENE TO INCREASE THE POTENCY OF INTRAVESICAL BCG IN TREATMENT OF BLADDER CANCER. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2009;2(3-4):1-8. Available from: https://sid.ir/paper/132594/en

IEEE: Copy

A. FEYZ BARAZANDEH, H. KHANAHMAD, GH.H. EBRAHIMIPOUR, M. ABOU ALHASANI, H.A.D. MOVASAGH, and T. SOHRABI, “DESIGN AND CONSTRUCTION A RECOMBINANT BCG CONTAINING CYTOSINE DEAMINASE SUICIDE GENE TO INCREASE THE POTENCY OF INTRAVESICAL BCG IN TREATMENT OF BLADDER CANCER,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 2, no. 3-4, pp. 1–8, 2009, [Online]. Available: https://sid.ir/paper/132594/en

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