CLONING, EXPRESSION AND PURIFICATION OF PSEUDOMONAS AERUGINOSA PILIN PROTEIN IN THE PROKARYOTIC HOST

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

KORPI FATEMEH; BEHROUZ BAHADOR; MOTAMEDIFAR MOHAMMAD; IRAJIAN GHOLAMREZA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY Year:2016 | Volume:9 | Issue:4 Page(s): 55-62

Download Full-Text

Persian Verion

View:

761

Download:

Cites:

Information Journal Paper

Title

CLONING, EXPRESSION AND PURIFICATION OF PSEUDOMONAS AERUGINOSA PILIN PROTEIN IN THE PROKARYOTIC HOST

Author(s)

KORPI FATEMEH | BEHROUZ BAHADOR | MOTAMEDIFAR MOHAMMAD | IRAJIAN GHOLAMREZA | Issue Writer Certificate

Keywords

PSEUDOMONAS AERUGINOSA PILIN PROTEINQ2

CLONINGQ3

EXPRESSIONQ3

Abstract

Background and Aim: Pathogenic Pseudomonas aeruginosa strains produce polar pili has required for motility, adhesion, and invasion. The main aims of the present study are to identify, clone, express and purify the recombinant pilin protein of P. aeruginosa in the prokaryotic host.Materials and Methods: The recombinant pilin gene (pilA) was isolated from P. aeruginosa PAO1 strain by PCR and cloned into pET-22b vector. The recombinant plasmid was subsequently veriﬁed by restriction analysis, and DNA sequencing. The recombinant vector was transformed into E. coli BL21 (DE3) strain, then the recombinant pilin overexpressed and affinity purified by Ni-NTA agarose-affinity chromatography. Western blot analysis was performed using anti-6His tag antibody.Results: The PCR and enzymatic digestion results showed the accuracy of the pilA gene CLONING. The protein electrophoresis showed that the molecular weight of recombinant pilin is about 19 kDa. Western blot analysis also confirmed the production of recombinant protein. The amount of produced protein was measured by the direct spectrophotometery method, which was 2.58 mg/mL.Conclusions: Western blot and ELISA results along with that of sequencing ensure accurate production of recombinant pilin, retaining its partial epitopes.

Cites

Protective Efficacy of a Divalent Candidate Vaccine Consisting of Type A Flagellin and Pilin Against Dermal Pseudomonas aeruginosa Infection in a Murine Burn Model

References

No record.

Cite

APA: Copy

KORPI, FATEMEH, BEHROUZ, BAHADOR, MOTAMEDIFAR, MOHAMMAD, & IRAJIAN, GHOLAMREZA. (2016). CLONING, EXPRESSION AND PURIFICATION OF PSEUDOMONAS AERUGINOSA PILIN PROTEIN IN THE PROKARYOTIC HOST. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 9(4), 55-62. SID. https://sid.ir/paper/132645/en

Vancouver: Copy

KORPI FATEMEH, BEHROUZ BAHADOR, MOTAMEDIFAR MOHAMMAD, IRAJIAN GHOLAMREZA. CLONING, EXPRESSION AND PURIFICATION OF PSEUDOMONAS AERUGINOSA PILIN PROTEIN IN THE PROKARYOTIC HOST. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2016;9(4):55-62. Available from: https://sid.ir/paper/132645/en

IEEE: Copy

FATEMEH KORPI, BAHADOR BEHROUZ, MOHAMMAD MOTAMEDIFAR, and GHOLAMREZA IRAJIAN, “CLONING, EXPRESSION AND PURIFICATION OF PSEUDOMONAS AERUGINOSA PILIN PROTEIN IN THE PROKARYOTIC HOST,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 9, no. 4, pp. 55–62, 2016, [Online]. Available: https://sid.ir/paper/132645/en

Related Journal Papers