ISOLATION AND MOLECULAR IDENTIFICATION OF MYCOPLASMA PULMONIS FROM THE RESPIRATORY TRACT OF RATTUS RATTUS

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SHAFAATI MOHAMMAD REZA; KHADEM NEDA SADAT; YAZDANSETAD SAJJAD; MOMENI TANAZ; ZARDADI MINA

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Journal: IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY Year:2016 | Volume:9 | Issue:4 Page(s): 24-31

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Title

ISOLATION AND MOLECULAR IDENTIFICATION OF MYCOPLASMA PULMONIS FROM THE RESPIRATORY TRACT OF RATTUS RATTUS

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Keywords

ISOLATIONQ2

MYCOPLASMA PULMONISQ4

RATSQ2

16SRRNAQ2

Abstract

Background and Aim: Mycoplasma is the smallest self-replicating bacteria polymorphic are having a diameter of about 200-500 nm, and lees cell wall. Because of their small size and flexibility to easily pass through the filter membrane and contaminate represented. These organisms are unique in nature, the wide range of diseases among animals, especially respiratory & genitalia diseases were among the rodents. The aim this study was to isolate and identify the molecule M. pulmonis of RATS.Materials and Methods: With aim to ISOLATION of M. pulmoni, the tracheal samples were collected and cultured on the medium 243 and Microaerophilic was placed. Genomic DNA was isolated from bacteria grown. 16SRRNA gene amplified with PCR. The PCR products, finally to identify the bacteria was purified, sequenced were a BLAST.Results: Macroscopic studies on the isolated colonies showed fried egg colonies were established. Based on the 16SRRNA sequencing studies, this bacterium belonging Mycoplasma genus & had 99% similarity to M. pulmoni.Conclusions: Due to the lack of similarity of 100%, a new strain of bacterium, M. pulmoni strain IRMT179 name were called. Finally, the gene bank was recorded with acceptation number KP836312. This study is the first time in Iran; M. pulmoni was isolated from the respiratory tract an urban rat.

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APA: Copy

SHAFAATI, MOHAMMAD REZA, KHADEM, NEDA SADAT, YAZDANSETAD, SAJJAD, MOMENI, TANAZ, & ZARDADI, MINA. (2016). ISOLATION AND MOLECULAR IDENTIFICATION OF MYCOPLASMA PULMONIS FROM THE RESPIRATORY TRACT OF RATTUS RATTUS. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 9(4), 24-31. SID. https://sid.ir/paper/132650/en

Vancouver: Copy

SHAFAATI MOHAMMAD REZA, KHADEM NEDA SADAT, YAZDANSETAD SAJJAD, MOMENI TANAZ, ZARDADI MINA. ISOLATION AND MOLECULAR IDENTIFICATION OF MYCOPLASMA PULMONIS FROM THE RESPIRATORY TRACT OF RATTUS RATTUS. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2016;9(4):24-31. Available from: https://sid.ir/paper/132650/en

IEEE: Copy

MOHAMMAD REZA SHAFAATI, NEDA SADAT KHADEM, SAJJAD YAZDANSETAD, TANAZ MOMENI, and MINA ZARDADI, “ISOLATION AND MOLECULAR IDENTIFICATION OF MYCOPLASMA PULMONIS FROM THE RESPIRATORY TRACT OF RATTUS RATTUS,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 9, no. 4, pp. 24–31, 2016, [Online]. Available: https://sid.ir/paper/132650/en

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