RAPID DِِIAGNOSIS OF LISTERIA MONOCYTOGENES BY PCR METHOD WITH HLYA GENE

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NAJAFI ALI; GHORBAN ALIZADEGAN M.; TAVAKOLI HAMID REZA; AHMADI ALI

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Journal: IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY Year:2009 | Volume:3 | Issue:2-3 Page(s): 9-14

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Title

RAPID DِِIAGNOSIS OF LISTERIA MONOCYTOGENES BY PCR METHOD WITH HLYA GENE

Author(s)

NAJAFI ALI | GHORBAN ALIZADEGAN M. | TAVAKOLI HAMID REZA | AHMADI ALI | Issue Writer Certificate

Keywords

LISTERIA MONOCYTOGENESQ4

PCRQ2

HLYA GENEQ4

Abstract

Background and Objectives: Currently the conventional method for the diagnosis of LISTERIA MONOCYTOGENES is culture method. However, the isolation rate is reduced in culture due to animals' food which is contains antibiotics. Besides, culture method is time consuming and takes more than 36 hrs. So more precise and rapid technique is needed. The aim of this study was application of PCR technique based on HLYA GENE fragment for rapid detection of LISTERIA MONOCYTOGENES in clinical samples.Materials and Methods: HLYA GENE was selected as a specific target sequencefor detection of LISTERIA MONOCYTOGENES. LISTERIA MONOCYTOGENES PTCC1163 was used as a standard organism for optimization experiments. A range of bacterial pathogens was used for specificity test including Escherichia coli: ATCC; 35218 Salmonella typhi PTCC: 1609. Phenol–chloroform method was used for DNA extraction. PCR technique was performed as per standard protocol. Amplified product was detected by 1% gel agarose electrophoresis, stained by ethidiome bromide.Results: Provided data confirmed amplification of expected product. Specificity test proved no cross reaction with tested organisms. Sensitivity test detected 500fg LISTERIA MONOCYTOGENES DNA as a final detection limit.Conclusion: Optimized experiment confirmed that the applied PCR protocol is quite fast with high sensitivity and specificity performing in less than three hours. Application of this method in clinical laboratories can help the rapid diagnosis of LISTERIA MONOCYTOGENES in samples.

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APA: Copy

NAJAFI, ALI, GHORBAN ALIZADEGAN, M., TAVAKOLI, HAMID REZA, & AHMADI, ALI. (2009). RAPID DِِIAGNOSIS OF LISTERIA MONOCYTOGENES BY PCR METHOD WITH HLYA GENE. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, 3(2-3), 9-14. SID. https://sid.ir/paper/132689/en

Vancouver: Copy

NAJAFI ALI, GHORBAN ALIZADEGAN M., TAVAKOLI HAMID REZA, AHMADI ALI. RAPID DِِIAGNOSIS OF LISTERIA MONOCYTOGENES BY PCR METHOD WITH HLYA GENE. IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY[Internet]. 2009;3(2-3):9-14. Available from: https://sid.ir/paper/132689/en

IEEE: Copy

ALI NAJAFI, M. GHORBAN ALIZADEGAN, HAMID REZA TAVAKOLI, and ALI AHMADI, “RAPID DِِIAGNOSIS OF LISTERIA MONOCYTOGENES BY PCR METHOD WITH HLYA GENE,” IRANIAN JOURNAL OF MEDICAL MICROBIOLOGY, vol. 3, no. 2-3, pp. 9–14, 2009, [Online]. Available: https://sid.ir/paper/132689/en

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