Synergistic Effect of Lovastatin and Selegiline on the Differentiation of Bone Marrow Stromal Cells Into Neuron-Like Cells

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Abdanipour Alireza; Karami Behnam; Fridoni Mohammad Javad; Mohamadi Momeneh; Fakheri Farzaneh

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Jentashapir Journal of Cellular and Molecular Biology Year:2023 | Volume:14 | Issue:4 Page(s): 1-8

Download Full-Text

View:

Download:

Cites:

Information Journal Paper

Title

Synergistic Effect of Lovastatin and Selegiline on the Differentiation of Bone Marrow Stromal Cells Into Neuron-Like Cells

Author(s)

Keywords

SelegilineQ4

LovastatinQ4

BMSCsQ4

NestinQ4

NF-68Q4

Abstract

Background: The effect of Selegiline as an oxidase inhibitor on cell differentiation into neuron-like cells has been demonstrated by altering gene expression. Based on the results of studies on the role of statins in neurotrophin regulation, in this study, we examined the effect of Lovastatin (HMG-CoA reductase inhibitor) on the differentiation of bone marrow mesenchymal cells (BMSCs) into neuron-like cells. Selegiline is an irreversible inhibitor of monoamine oxidase (MAO) type B. Since dopamine in the human brain is metabolized primarily by MAO-B, Selegiline increases dopamine levels in the central nervous system. In addition to inhibiting MAO-B, Selegiline also inhibits the uptake of dopamine and norepinephrine into presynaptic nerves and increases dopamine turnover. Methods: Bone marrow mesenchymal cells were collected from 28-day-old rats and cultured under standard conditions on the medium. The experimental groups in this study were as follows: BMSCs (control),BMSCs induced with 20 µM Selegiline for 24 hours (experiment 1),BMSCs induced with 6 µM Lovastatin for 24 hours (experiment 2),BMSCs were induced with 20 µM Selegiline for 24 hours and 6 µM Lovastatin for the next 24 hours (experiment 3). Real-time RT-PCR was performed to determine the mRNA levels of the Nestin and NF-68 genes. Results: Real-time RT-PCR results showed that Nestin and NF-68 mRNA levels were significantly increased in the co-treatment group (experiment 3) compared to the other experimental groups (P < 0. 05). Conclusions: Based on the increased expression of Nestin and NF-68 genes, the presence of Lovastatin has a synergistic effect on neuronal differentiation and optimization of stem cell therapeutic approaches.

Cites

No record.

References