Analyzing Antibiotic Resistance in Clinical Mycobacterium tuberculosis Isolates using Microplate Alamar Blue Assay

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Ahmadi Kamal; Madadi Goli Nahid; Masoumi Morteza; Nasehi Mahshid; Siadat Seyed Davar; Vaziri Farzam; Fateh Abolfazl

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Journal Paper

Paper Information

Journal: Journal of Medical Microbiology and Infectious Diseases Year:2023 | Volume:11 | Issue:4 Page(s): 174-178

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Title

Analyzing Antibiotic Resistance in Clinical Mycobacterium tuberculosis Isolates using Microplate Alamar Blue Assay

Author(s)

Keywords

Mycobacterium tuberculosisQ4

BedaquilineQ4

Microplate Alamar Blue AssayQ4

Multi-drug resistanceQ4

Extensive drug-resistant tuberculosisQ4

Abstract

Introduction: Tuberculosis, caused by Mycobacterium tuberculosis, is one of the most common infectious diseases worldwide. Epidemiological studies of M. tuberculosis drug resistance are critical for improving patient treatment approaches and controlling the spread of tuberculosis. The present study aimed to determine antibiotic resistance among M. tuberculosis clinical isolates using the Microplate Alamar Blue Assay (MABA). Methods: In this descriptive cross-sectional study, 25 M. tuberculosis isolates from clinical samples were identified and confirmed using standard microbiological and biochemical tests. Then, the MIC for the antibiotics Bedaquiline, isoniazid, rifampin, ethambutol, ofloxacin, moxifloxacin, capreomycin, and streptomycin was determined using the MABA method. The results were analyzed using SPSS version 16 software. Results: Among the 25 investigated isolates, the frequencies for MDR, Pre-XDR, and XDR isolates were 20%, 8%, and 32%, respectively. The highest rate of drug resistance was to isoniazid (80%), rifampicin, and ethambutol (76%), and the highest rate of sensitivity was to moxifloxacin (68%). The frequency of isoniazid mono-resistance and rifampicin mono-resistance was 5 cases (50%) and 4 cases (40%), respectively. Conclusion: Our study revealed an alarming rate of MDR and XDR M. tuberculosis strains, indicating that current first-line treatments may be ineffective for a significant number of patients. The Bedaquiline resistance among the isolates with no history of previous exposure to this drug suggests unexplored resistance mechanisms. Molecular techniques to accurately identify these mechanisms may contribute to developing more effective treatment strategies to combat drug-resistant tuberculosis.

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