Investigation of biofilm formation and virulence factors in Pseudomonas aeruginosa isolates from urinary tract infection patients in Tehran hospitals

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Shahbazi Shahla; Rahimzadeh Mohammad; Habibi Mehri; Azizi Omid; Asadi Karam Mohammad Reza

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Journal of Torbat Heydariyeh University of Medical Sciences Year:2024 | Volume:12 | Issue:2 Page(s): 1-15

Download Full-Text

Persian Verion

View:

Download:

Cites:

Information Journal Paper

Title

Investigation of biofilm formation and virulence factors in Pseudomonas aeruginosa isolates from urinary tract infection patients in Tehran hospitals

Author(s)

Keywords

Pseudomonas aeruginosaQ4

Urinary Tract InfectionQ4

BiofilmQ4

VirulenceQ4

Abstract

Background & Aim:Virulence factors play a crucial role in the ability of Pseudomonas aeruginosa to cause Urinary Tract Infections. This study aimed to identify Virulence genes in clinical isolates of P. aeruginosa from two hospitals in Tehran, Iran. Methods: In this study, 200 P. aeruginosa isolates were collected from Urinary Tract Infections in two general hospitals in Tehran, Iran. The isolates were confirmed using standard laboratory tests. Biofilm formation ability was assessed using a phenotypic method. Furthermore, genes encoding selected Virulence factors were identified using polymerase chain reaction (PCR). Results: 53% of the collected isolates exhibited strong Biofilm production. The presence of algD, lasB, pcrV, aprA, plcN, toxA, exoS, pilB, plcH and exoU genes was observed in 90%, 88%, 85%, 84%, 80%, 77%, 66%, 62%, 58%, and 45% of the isolates, respectively. A significant association was observed between the presence of the exoS Virulence gene and patient age (P=0.04). Furthermore, a significant association was observed between the presence of all investigated genes (except plcN and toxA) and the type of hospital. When investigating the relationship between Virulence gene type and Biofilm formation intensity, a significant association was observed between the presence of the exoS gene and Biofilm formation capacity (p=0.03). Conclusion: This study demonstrated that P. aeruginosa isolated from Urinary Tract Infections possess Virulence genes and a high capacity for Biofilm formation. This finding suggests that P. aeruginosa could pose significant challenges in treatment and increase the risk of infection and mortality in patients.

Cites

No record.

References