PRODUCTION AND PURIFICATION OF A PROTEASE FROM AN ALKALOPHILIC BACILLUS SP. 2-5 STRAIN ISOLATED FROM SOIL

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FALAHAT PISHEH H.R.; KHOSRAVI DARANI KIANOUSH; JALALI M.; BADAMI N.; MARDANI N.

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Journal: Iranian Journal of Biotechnology Year:2007 | Volume:5 | Issue:2 Page(s): 110-113

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Title

PRODUCTION AND PURIFICATION OF A PROTEASE FROM AN ALKALOPHILIC BACILLUS SP. 2-5 STRAIN ISOLATED FROM SOIL

Author(s)

Keywords

ALKALOPHILIC BACILLUSQ3

PROTEASEQ2

PROCESS VARIABLESQ3

PURIFICATIONQ3

Abstract

This research has focused on isolation and characterization of a strain of Bacillus sp. from alkaline soil, which was able to produce extracellular alkaline PROTEASE at pHs ranging from 8 to 11 and temperatures of 20 to 50°C. Also the impact of different carbon and nitrogen sources were investigated. The yield and fold of enzyme PURIFICATION was 24% and 50 times, respectively. Molecular weight of purified enzyme was measured by SDS-PAGE as 24.7 kDa. The alkaline PROTEASE produced by Bacillus sp. 2 - 5 showed the most caseinolytic activity (without any gelatinolytic activity) at pH>10.

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APA: Copy

FALAHAT PISHEH, H.R., KHOSRAVI DARANI, KIANOUSH, JALALI, M., BADAMI, N., & MARDANI, N.. (2007). PRODUCTION AND PURIFICATION OF A PROTEASE FROM AN ALKALOPHILIC BACILLUS SP. 2-5 STRAIN ISOLATED FROM SOIL. IRANIAN JOURNAL OF BIOTECHNOLOGY, 5(2), 110-113. SID. https://sid.ir/paper/139230/en

Vancouver: Copy

FALAHAT PISHEH H.R., KHOSRAVI DARANI KIANOUSH, JALALI M., BADAMI N., MARDANI N.. PRODUCTION AND PURIFICATION OF A PROTEASE FROM AN ALKALOPHILIC BACILLUS SP. 2-5 STRAIN ISOLATED FROM SOIL. IRANIAN JOURNAL OF BIOTECHNOLOGY[Internet]. 2007;5(2):110-113. Available from: https://sid.ir/paper/139230/en

IEEE: Copy

H.R. FALAHAT PISHEH, KIANOUSH KHOSRAVI DARANI, M. JALALI, N. BADAMI, and N. MARDANI, “PRODUCTION AND PURIFICATION OF A PROTEASE FROM AN ALKALOPHILIC BACILLUS SP. 2-5 STRAIN ISOLATED FROM SOIL,” IRANIAN JOURNAL OF BIOTECHNOLOGY, vol. 5, no. 2, pp. 110–113, 2007, [Online]. Available: https://sid.ir/paper/139230/en

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