THE PRODUCTION OF ALKALINE PROTEASE BY BACILLUS TEQUILENSIS FJSH2 ISOLATED FROM JIROFT’S SLAUGHTERHOUSE WASTES

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BADOEI DALFARD ARASTOO; AMIRI PARVIN; MEZANIPOUR NARJES; KARAMI ZAHRA; GHANBARI BATOOL

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Journal: JOURNAL OF MICROBIAL WORLD Year:2014 | Volume:8 | Issue:1 (22) Page(s): 54-63

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Title

THE PRODUCTION OF ALKALINE PROTEASE BY BACILLUS TEQUILENSIS FJSH2 ISOLATED FROM JIROFT’S SLAUGHTERHOUSE WASTES

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Keywords

ALKALINE PROTEASEQ2

WASTESQ2

SCREENINGQ2

BACILLUS TEQUILENSISQ2

Abstract

Background & Objectives: Proteases are the most applied industrial enzymes usable in several biotechnological industries. These enzymes include approximately 60% of enzyme marketing worldwide. ALKALINE PROTEASEs are the most important enzymes which are stable in the presence of alkaline pH, surfactant and oxidizing agents. This study was aimed to isolate and identify the protease producing bacteria from slaughterhouse WASTES in Jiroft.Materials & Methods: This cross - ec ional ud wa per ormed on Jiro’ laug er ou e WASTES. The protease producing strain was screened on the skim milk agar media. The 16S rRNA genes of the samples were obtained using PCR and sequencing. The protease enzyme was partially purified by ammonium sulphate precipitation, dialysis and ion exchange chromatography. Thereafter, The biochemical features of the enzymes were analysed.Results: The gene sequencing study showed that the isolated strain were 100 % similar to BACILLUS TEQUILENSIS species. The purified protease showed maximum activity and stability in pH 9 and 40°C. Km and Vmax values were obtained based on Linewewr berg equation 6.77 mg/ml and 64.94 U/ml/min, respectively. Protease stability was improved about 1.8 and 2.4 folds in the presence of cyclohexane and DMSO, respectively.Conclusion: The activity and stability of this enzyme in alkaline pH and in the presence of organic solvents indicates its potential to be used in industries.

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APA: Copy

BADOEI DALFARD, ARASTOO, AMIRI, PARVIN, MEZANIPOUR, NARJES, KARAMI, ZAHRA, & GHANBARI, BATOOL. (2014). THE PRODUCTION OF ALKALINE PROTEASE BY BACILLUS TEQUILENSIS FJSH2 ISOLATED FROM JIROFT’S SLAUGHTERHOUSE WASTES. JOURNAL OF MICROBIAL WORLD, 8(1 (22)), 54-63. SID. https://sid.ir/paper/189319/en

Vancouver: Copy

BADOEI DALFARD ARASTOO, AMIRI PARVIN, MEZANIPOUR NARJES, KARAMI ZAHRA, GHANBARI BATOOL. THE PRODUCTION OF ALKALINE PROTEASE BY BACILLUS TEQUILENSIS FJSH2 ISOLATED FROM JIROFT’S SLAUGHTERHOUSE WASTES. JOURNAL OF MICROBIAL WORLD[Internet]. 2014;8(1 (22)):54-63. Available from: https://sid.ir/paper/189319/en

IEEE: Copy

ARASTOO BADOEI DALFARD, PARVIN AMIRI, NARJES MEZANIPOUR, ZAHRA KARAMI, and BATOOL GHANBARI, “THE PRODUCTION OF ALKALINE PROTEASE BY BACILLUS TEQUILENSIS FJSH2 ISOLATED FROM JIROFT’S SLAUGHTERHOUSE WASTES,” JOURNAL OF MICROBIAL WORLD, vol. 8, no. 1 (22), pp. 54–63, 2014, [Online]. Available: https://sid.ir/paper/189319/en

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