Isolation and identification of alkaline protease-producing bacterium in the presence of washing-powder from Geinarje hot spring, Ardabil, Iran

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Nasre Taheri Mahnaz; Ebrahimipour Gholamhosein; SADEGHI HOSEIN

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Journal: NOVA BIOLOGICA REPERTA Year:2019 | Volume:6 | Issue:3 Page(s): 292-301

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Title

Isolation and identification of alkaline protease-producing bacterium in the presence of washing-powder from Geinarje hot spring, Ardabil, Iran

Author(s)

Nasre Taheri Mahnaz | Ebrahimipour Gholamhosein | SADEGHI HOSEIN | Issue Writer Certificate

Keywords

alkaline proteaseQ1

Brevibacillus sp.Q2

activityQ2

stabilityQ2

laundry detergentQ2

Abstract

Proteases are important industrial enzymes used in different areas of industry, mainly detergent, food and leather industries. In this study, novel alkaline protease-producing bacterium was isolated from Geinarje hot spring and examined for maximum protease activity to be utilized in washing-powder. The isolated bacterium was cultured in mineral salt medium including 2% Skim Milk. Proteolytic activity of supernatant was measured by caseinolytic method. The effects of pH, temperature, SDS, Tween 80 and EDTA on protease stability and activity were investigated. The detergent compatibility of protease was assayed. On the basis of phylogenetic analysis and morphological as well as biochemical tests, the isolate was identified as a new strain of Brevibacillus borstelensis capable of generating extracellular alkaline protease. The generated protease was determined as alkaline metallo-protease having high activity at 60 oC and pH 9. Moreover, the alkaline protease was stable in the presence of SDS, Tween 80 and H2O2. It is compatible with commercial detergents. Finding proteases capable of degrading proteins in extreme environment (i. e. alkaline pH, high temperature and presence of surfactants) is valuable in biotechnological and industrial practices. Therefore, it can be utilized in detergent formulation in the future.

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APA: Copy

Nasre Taheri, Mahnaz, Ebrahimipour, Gholamhosein, & SADEGHI, HOSEIN. (2019). Isolation and identification of alkaline protease-producing bacterium in the presence of washing-powder from Geinarje hot spring, Ardabil, Iran. NOVA BIOLOGICA REPERTA, 6(3 ), 292-301. SID. https://sid.ir/paper/250441/en

Vancouver: Copy

Nasre Taheri Mahnaz, Ebrahimipour Gholamhosein, SADEGHI HOSEIN. Isolation and identification of alkaline protease-producing bacterium in the presence of washing-powder from Geinarje hot spring, Ardabil, Iran. NOVA BIOLOGICA REPERTA[Internet]. 2019;6(3 ):292-301. Available from: https://sid.ir/paper/250441/en

IEEE: Copy

Mahnaz Nasre Taheri, Gholamhosein Ebrahimipour, and HOSEIN SADEGHI, “Isolation and identification of alkaline protease-producing bacterium in the presence of washing-powder from Geinarje hot spring, Ardabil, Iran,” NOVA BIOLOGICA REPERTA, vol. 6, no. 3 , pp. 292–301, 2019, [Online]. Available: https://sid.ir/paper/250441/en

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