DETERMINATION OF POLYCYCLIC AROMATIC HYDROCARBONS IN WATER USING AN ELECTROCHEMICAL DNA BIOSENSOR

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MANAFFAR R.; SEHATNIA B.; SABZI R.; EHSANI A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF HEALTH AND ENVIRONMENT Year:2015 | Volume:8 | Issue:2 Page(s): 225-236

Download Full-Text

Persian Verion

View:

1,397

Download:

Cites:

Information Journal Paper

Title

DETERMINATION OF POLYCYCLIC AROMATIC HYDROCARBONS IN WATER USING AN ELECTROCHEMICAL DNA BIOSENSOR

Author(s)

MANAFFAR R. | SEHATNIA B. | SABZI R. | EHSANI A. | Issue Writer Certificate

Keywords

POLYCYCLIC AROMATIC HYDROCARBONS (PAHS)Q2

ELECTROCHEMICAL BIOSENSORQ2

LIQUID-LIQUID CHROMATOGRAPHYQ2

SCREEN-PRINTED ELECTRODEQ2

Abstract

Background and Objectives: Polycyclic aromatic hydrocarbons (PAHs) are widespread environmental contaminants in aquatic environments. These contaminants are generated through oil spills, manufactory processes, and industrial wastes or naturally through the incomplete combustion of coal, oil, gas, and wood waste. Most of these compounds are noted as carcinogenic and mutagenic. Therefore, detection of these pollutants by a sensitive and inexpensive method is very important.Materials and Methods: In this study, an electrochemical DNA biosensor was used to detect PAHs due to its sensitivity, ability, and high response rate. For this purpose, the bovine thymus double-stranded DNA was fixed on a SCREEN-PRINTED ELECTRODE. Then, the electrodes electrochemical behavior was investigated.This electrochemical DNA biosensor works upon the difference between the electrochemical response of guanine bases in DNA structure in the presence and absence of PAH compounds. To evaluate the biosensors performance, the response of biosensor to real samples was compared with conventional pollutant determination methods like LIQUID-LIQUID CHROMATOGRAPHY.Results: Optimum conditions were examined for biosensor response including effect of activation potential and time on electrode pretreatment, applied potential for DNA immobilization, and detection potential.Under optimal conditions, the pretreatment of the electrode obtained in 1.6 V for 350s, then the DNA was immobilized on the electrode surface by applying a potential of -0.5 V to detect different PAHs in real samples in the range of micro molar.Conclusion: Electrochemical DNA biosensors are capable of detecting the sum of PAHs in water samples with high accuracy, sensitivity, and low cost compared with chromatographic methods.

Cites

No record.

References

Cite

APA: Copy

MANAFFAR, R., SEHATNIA, B., SABZI, R., & EHSANI, A.. (2015). DETERMINATION OF POLYCYCLIC AROMATIC HYDROCARBONS IN WATER USING AN ELECTROCHEMICAL DNA BIOSENSOR. IRANIAN JOURNAL OF HEALTH AND ENVIRONMENT, 8(2), 225-236. SID. https://sid.ir/paper/145945/en

Vancouver: Copy

MANAFFAR R., SEHATNIA B., SABZI R., EHSANI A.. DETERMINATION OF POLYCYCLIC AROMATIC HYDROCARBONS IN WATER USING AN ELECTROCHEMICAL DNA BIOSENSOR. IRANIAN JOURNAL OF HEALTH AND ENVIRONMENT[Internet]. 2015;8(2):225-236. Available from: https://sid.ir/paper/145945/en

IEEE: Copy

R. MANAFFAR, B. SEHATNIA, R. SABZI, and A. EHSANI, “DETERMINATION OF POLYCYCLIC AROMATIC HYDROCARBONS IN WATER USING AN ELECTROCHEMICAL DNA BIOSENSOR,” IRANIAN JOURNAL OF HEALTH AND ENVIRONMENT, vol. 8, no. 2, pp. 225–236, 2015, [Online]. Available: https://sid.ir/paper/145945/en

Related Journal Papers