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Information Journal Paper

Title

IN VITRO EVALUATION OF ANTI-LEISHMANIAL ACTIVITIES OF ZATARIA MULTIFLORA BOISS, PEGANUM HARMALA AND MYRTUS COMMUNIS BY COLORIMETRIC ASSAY

Pages

  32-41

Abstract

 Background & Aims: PLANT EXTRACTS and plant-derived compounds are valuable sources of new medicinal agents that are commonly used to treat infectious diseases. The purpose of this study was to evaluate, anti- Leishmanial activity of three PLANT EXTRACTS on L.major promastigotes by colorimetric assay as compared to a trivalent antimonial compound (tartar emetic). Methods: LEISHMANIA MAJOR promastigotes were cultured at 25±2°C in stationary phases in RPMI-1640 medium supplemented with 10% heat inactivated fetal calf serum (FCS) and antibiotic. Then by using MTT assay, the biological activity of PLANT EXTRACTS in comparison to potassium antimonyl tartrate [Sb (III)] on L.major promastigotes were assessed. The optical density (OD) due to cleavage of the tetrazolium salt MTT [3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide] into a colored product formazan by the parasite was measured using ELISA reader and IC50 values (50% inhibitory concentrations) were determined. All experiments were repeated in duplicate.Results: PLANT EXTRACTS and Tartar emetic inhibited the growth of promastigote forms of L.major in vitro after 72 hours of incubation. IC50 of tartar emetic was 4.7 mg/ml, and IC50 values for ZATARIA MULTIFLORA BOISS, PEGANUM harmala and Myrtus communis extracts were 7.4, 7.2 and 5.8 mg/ml, respectively. Although tartar emetic was more effective than PLANT EXTRACTS, all extracts had profound effects on promastigotes of L.major.Conclusion: Since, PLANT EXTRACTS of ZATARIA MULTIFLORA BOISS, PEGANUM harmala and Myrtus communis have anti-Leishmanial effects in vitro, further works are required to evaluate the exact effect of these extracts on Leishmania agents in animal models.

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  • Cite

    APA: Copy

    BARATI, MOHAMMAD, SHARIFI, I., & SHARIFIFAR, FARIBA. (2010). IN VITRO EVALUATION OF ANTI-LEISHMANIAL ACTIVITIES OF ZATARIA MULTIFLORA BOISS, PEGANUM HARMALA AND MYRTUS COMMUNIS BY COLORIMETRIC ASSAY. JOURNAL OF KERMAN UNIVERSITY OF MEDICAL SCIENCES, 17(1), 32-41. SID. https://sid.ir/paper/146/en

    Vancouver: Copy

    BARATI MOHAMMAD, SHARIFI I., SHARIFIFAR FARIBA. IN VITRO EVALUATION OF ANTI-LEISHMANIAL ACTIVITIES OF ZATARIA MULTIFLORA BOISS, PEGANUM HARMALA AND MYRTUS COMMUNIS BY COLORIMETRIC ASSAY. JOURNAL OF KERMAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2010;17(1):32-41. Available from: https://sid.ir/paper/146/en

    IEEE: Copy

    MOHAMMAD BARATI, I. SHARIFI, and FARIBA SHARIFIFAR, “IN VITRO EVALUATION OF ANTI-LEISHMANIAL ACTIVITIES OF ZATARIA MULTIFLORA BOISS, PEGANUM HARMALA AND MYRTUS COMMUNIS BY COLORIMETRIC ASSAY,” JOURNAL OF KERMAN UNIVERSITY OF MEDICAL SCIENCES, vol. 17, no. 1, pp. 32–41, 2010, [Online]. Available: https://sid.ir/paper/146/en

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