DETECTING THE ASPERGILLUS SPP. IN (BAL) FLUID SAMPLES BY NESTED PCR, CULTURE AND DIRECT SMEAR

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YAZDANPARAST SEYED AMIR; GHANDCHI GHAZALEH; HESHMATI FARIBA; AFSHAR MOGHADDAM SANAM; KHODADOUST MOHAMMAD ALI

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Title

DETECTING THE ASPERGILLUS SPP. IN (BAL) FLUID SAMPLES BY NESTED PCR, CULTURE AND DIRECT SMEAR

Author(s)

Keywords

ASPERGILLUS SPPQ3

DIRECT SMEARQ3

NESTED PCRQ3

FUNGAL CULTUREQ3

Abstract

Background and Aim: Aspergillosis is the most prevalent cause of the respiratory infections. These fungi show invasive aspergillosis (IA) in immunocompromised patients. The numbers of immunocompromised patients are increasing due to immunodisorder illnesses, grafts and immunosuppressor drugs, so, rapid identification methods are very important.The aim of this study was to detect the ASPERGILLUS SPP. In fluid samples by NESTED PCR, and compare with culture and DIRECT SMEAR.Materials and Methods: Conventional detection methods such as culture and DIRECT SMEAR are insensitive and time consuming. Some methods such as immunodetecting methods have high false positive and are unreliable.Nowadays, molecular methods and PCR are very helpful. These methods are both sensitive and reliable and very rapid. In this study, we used NESTED PCR, culture and DIRECT SMEAR to detect Aspergillums spp in BAL fluid samples.Results: This research is a descriptive-comparative study and has been done for rapid identification of fungi related to Aspergillosis such as culture, DIRECT SMEAR and nested- PCR. Findings of this study show that positive results by nested-PCR were more effective and sensitive than culture and DIRECT SMEAR.Conclusions: We found that positive results by PCR were more effective and sensitive than two other methods.

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APA: Copy

YAZDANPARAST, SEYED AMIR, GHANDCHI, GHAZALEH, HESHMATI, FARIBA, AFSHAR MOGHADDAM, SANAM, & KHODADOUST, MOHAMMAD ALI. (2011). DETECTING THE ASPERGILLUS SPP. IN (BAL) FLUID SAMPLES BY NESTED PCR, CULTURE AND DIRECT SMEAR. PAYAVARD-SALAMAT, 5(3), 80-87. SID. https://sid.ir/paper/149636/en

Vancouver: Copy

YAZDANPARAST SEYED AMIR, GHANDCHI GHAZALEH, HESHMATI FARIBA, AFSHAR MOGHADDAM SANAM, KHODADOUST MOHAMMAD ALI. DETECTING THE ASPERGILLUS SPP. IN (BAL) FLUID SAMPLES BY NESTED PCR, CULTURE AND DIRECT SMEAR. PAYAVARD-SALAMAT[Internet]. 2011;5(3):80-87. Available from: https://sid.ir/paper/149636/en

IEEE: Copy

SEYED AMIR YAZDANPARAST, GHAZALEH GHANDCHI, FARIBA HESHMATI, SANAM AFSHAR MOGHADDAM, and MOHAMMAD ALI KHODADOUST, “DETECTING THE ASPERGILLUS SPP. IN (BAL) FLUID SAMPLES BY NESTED PCR, CULTURE AND DIRECT SMEAR,” PAYAVARD-SALAMAT, vol. 5, no. 3, pp. 80–87, 2011, [Online]. Available: https://sid.ir/paper/149636/en

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