ISOLATION AND DETECTION OF MYCOPLASMA SYNOVIAE FROM SEROPOSITIVE RAPID REACTION BROILER BREEDER FLOCKS BY POLYMERASE CHAIN REACTION AND CULTURE METHODS

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HAGHBIN NAZARPAK H.; POURBAKHSH S.A.; CHARKHKAR S.; SHEIKHI N.; ASHTARI A.

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Paper Information

Journal: JOURNAL OF VETERINARY MICROBIOLOGY Year:2010 | Volume:6 | Issue:1 (19) Page(s): 31-35

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Title

ISOLATION AND DETECTION OF MYCOPLASMA SYNOVIAE FROM SEROPOSITIVE RAPID REACTION BROILER BREEDER FLOCKS BY POLYMERASE CHAIN REACTION AND CULTURE METHODS

Author(s)

Keywords

MYCOPLASMA SYNOVIAEQ2

CULTUREQ2

PCRQ2

ISOLATIONQ2

Abstract

MYCOPLASMA SYNOVIAE (MS) is one of the main disease in fowl which cause infections of respiratory system and tenosynovitis. Characterization and successful detection of MS in broiler breeder farms help to prevent distribution of MS in broiler farms. The aim of this study was ISOLATION and characterization of MS in broiler breeder farms by CULTURE and polymerase chain reaction (PCR) techniques. Thirty specimens which obtained from flocks with positive serum reactions were studied by CULTURE and PCR techniques. After collecting of Choanal cleft and tracheal swabs in PPLO broth, a short-term incubation was occurred and then filtered and a serial passage was performed. In the fourth passage, all of the specimens CULTUREd on PPLO agar, in addition, original specimens used to extract of DNA and MS-PCR technique. Results showed that 15.30 were positive by CULTURE (Approved by using of standard MS specific antiserum) and 25.30 were positive by PCR. These observations highlight the higher sensitivity of PCR rather than CULTURE. These results may be observed because of PCR ability to detect DNA after mycoplasma’s death. However positive results of CULTURE obtained when there were not defect on swabbing and handling of specimens which can cause harmful effects on MS and accordingly suitable environment for growth of MS was exactly prepared.

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References

EXPERIMENTAL EVIDENCE OF INDIRECT TRANSMISSION OF MYCOPLASMA SYNOVIAE

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APA: Copy

HAGHBIN NAZARPAK, H., POURBAKHSH, S.A., CHARKHKAR, S., SHEIKHI, N., & ASHTARI, A.. (2010). ISOLATION AND DETECTION OF MYCOPLASMA SYNOVIAE FROM SEROPOSITIVE RAPID REACTION BROILER BREEDER FLOCKS BY POLYMERASE CHAIN REACTION AND CULTURE METHODS. JOURNAL OF VETERINARY MICROBIOLOGY, 6(1 (19)), 31-35. SID. https://sid.ir/paper/152016/en

Vancouver: Copy

HAGHBIN NAZARPAK H., POURBAKHSH S.A., CHARKHKAR S., SHEIKHI N., ASHTARI A.. ISOLATION AND DETECTION OF MYCOPLASMA SYNOVIAE FROM SEROPOSITIVE RAPID REACTION BROILER BREEDER FLOCKS BY POLYMERASE CHAIN REACTION AND CULTURE METHODS. JOURNAL OF VETERINARY MICROBIOLOGY[Internet]. 2010;6(1 (19)):31-35. Available from: https://sid.ir/paper/152016/en

IEEE: Copy

H. HAGHBIN NAZARPAK, S.A. POURBAKHSH, S. CHARKHKAR, N. SHEIKHI, and A. ASHTARI, “ISOLATION AND DETECTION OF MYCOPLASMA SYNOVIAE FROM SEROPOSITIVE RAPID REACTION BROILER BREEDER FLOCKS BY POLYMERASE CHAIN REACTION AND CULTURE METHODS,” JOURNAL OF VETERINARY MICROBIOLOGY, vol. 6, no. 1 (19), pp. 31–35, 2010, [Online]. Available: https://sid.ir/paper/152016/en

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