Investigating the Effect of Galbanic Acid on Lipin-1 and Lipin-2 Genes in Fatty Liver Cells with Palmitate

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Musavi Hadis; Iraie Reza; Mohammadi Maryam; Barartabar Zeinab; Yazdi Mohammad; Bagheri Abouzar; Khonakdar Tarsi Abbas

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Advanced Biomedical Research Year:621 | Volume: | Issue: Page(s): 1-7

Download Full-Text

View:

Download:

Cites:

Information Journal Paper

Title

Investigating the Effect of Galbanic Acid on Lipin-1 and Lipin-2 Genes in Fatty Liver Cells with Palmitate

Author(s)

Keywords

Galbanic acid; lipin-1; lipin-2; non-alcoholic fatty liver; resveratrol

Abstract

Background: Non-alcoholic fatty liver disease is related to lipid accumulation and inflammation. Considering the role of lipin-1 and lipin-2 in fat homeostasis and inflammation, this study aimed to explore the effect of galbanic acid (Gal) and resveratrol (RSV) on alterations in the gene expression levels and protein abundance of lipin-1 and lipin-2 in HepG2 liver cells lipid-enriched with palmitate (Pal). Materials and Methods: HepG2 cells were subjected to different amounts of Gal and RSV for 24 hours in the presence of Pal to induce lipid accumulation. The RT-PCR method was employed to assess the expression of lipin-1 and lipin-2 genes, while protein levels were evaluated by western blot analysis. Lipid accumulation was determined qualitatively and semi-quantitatively using the oil-red staining technique. Results: Gal treatment increased lipin-1 and lipin-2 gene expression (P < 0. 05). In contrast, the groups treated with RSV did not show a substantial variance in the expression levels of the two genes (P > 0. 05). In the groups treated with Gal/RSV, the intensity of lipin-2 protein bands was higher compared to the Pal group (P > 0. 01),however, the intensity of lipin-1 protein bands was not significantly different (P > 0. 05). Conclusion: Gal, a coumarin compound, significantly increased the expression of lipin-1 and lipin-2 in HepG2 cells treated with Pal. Consequently, this research suggests gal as a novel strategy for regulating fat homeostasis in HepG2 cells.

Multimedia

No record.

Cites

No record.

References