THE ROLE OF ULTRAWEB NANO-FIBRILLAR SUBSTRATES IN THE DIFFERENTIATION OF IN VITRO MOUSE BONE MARROW MESENCHYMAL STEM CELL-DERIVED HEPATOCYTE-LIKE CELLS

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PIRYAEI A.; REZAZADEH VALOUJERDI M.; SHAHSAVANI M.; BAHARVAND HOSSEIN

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Journal: Cell Journal (Yakhteh) Year:2010 | Volume:12 | Issue:2 (46) Page(s): 275-286

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Title

THE ROLE OF ULTRAWEB NANO-FIBRILLAR SUBSTRATES IN THE DIFFERENTIATION OF IN VITRO MOUSE BONE MARROW MESENCHYMAL STEM CELL-DERIVED HEPATOCYTE-LIKE CELLS

Author(s)

PIRYAEI A. | REZAZADEH VALOUJERDI M. | SHAHSAVANI M. | BAHARVAND HOSSEIN | Issue Writer Certificate

Keywords

MESENCHYMAL STEM CELLS (MSCS)Q1

DIFFERENTIATIONQ2

HEPATOCYTEQ2

NANOFIBERSQ1

Abstract

Objective: To study the impact of ultraweb nano-fibrillar substrates on in vitro DIFFERENTIATION of mouse bone marrow-derived mesenchymal stem cells into HEPATOCYTE-like cells.Materials and Methods: For a duration of up to 36 days, mouse bone marrow mesenchymal stem cells (MSCs) were cultured on an artificial basement membrane containing ultraweb nanofiber (nano+) and plates (nano-) coated with Matrigel and used growth and differentiating factors for hepatogenic DIFFERENTIATION. Then, to study the impact of NANOFIBERS on HEPATOCYTE DIFFERENTIATION, mRNA expressions for liver-specific genes (CK7, AFP, FOXa2, ALB, CYP1a1, HNF4a) were analyzed using real-time PCR , and ultrastructures of differentiated cells were evaluated using scanning and transmission electron microscope.Results: Both experimental groups expressed mRNA of liver-specific genes in a time dependent manner. However, mRNA expression trends of liver-specific genes, especially those of FOXa2 and HNF4a, were better in the nano+ culture as compared to the nano- culture (p<0.05). Furthermore, the ultrastructures of differentiated cells in the 36 day-old nano+culture were more mature as compared to those of the nano- group. Conclusion: The results suggest that topographical properties of the extracellular matrices produced by ultraweb NANOFIBERS are effective for the in vitro reproduction of more differentiated MSC-derived HEPATOCYTE-like cells.

Cites

THE EFFECT OF HUMAN BONE MARROW-MESENCHYMAL STEM CELLS SECRETOMS ON DIABETIC WOUND HEALING

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APA: Copy

PIRYAEI, A., REZAZADEH VALOUJERDI, M., SHAHSAVANI, M., & BAHARVAND, HOSSEIN. (2010). THE ROLE OF ULTRAWEB NANO-FIBRILLAR SUBSTRATES IN THE DIFFERENTIATION OF IN VITRO MOUSE BONE MARROW MESENCHYMAL STEM CELL-DERIVED HEPATOCYTE-LIKE CELLS. CELL JOURNAL (YAKHTEH), 12(2 (46)), 275-286. SID. https://sid.ir/paper/16195/en

Vancouver: Copy

PIRYAEI A., REZAZADEH VALOUJERDI M., SHAHSAVANI M., BAHARVAND HOSSEIN. THE ROLE OF ULTRAWEB NANO-FIBRILLAR SUBSTRATES IN THE DIFFERENTIATION OF IN VITRO MOUSE BONE MARROW MESENCHYMAL STEM CELL-DERIVED HEPATOCYTE-LIKE CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2010;12(2 (46)):275-286. Available from: https://sid.ir/paper/16195/en

IEEE: Copy

A. PIRYAEI, M. REZAZADEH VALOUJERDI, M. SHAHSAVANI, and HOSSEIN BAHARVAND, “THE ROLE OF ULTRAWEB NANO-FIBRILLAR SUBSTRATES IN THE DIFFERENTIATION OF IN VITRO MOUSE BONE MARROW MESENCHYMAL STEM CELL-DERIVED HEPATOCYTE-LIKE CELLS,” CELL JOURNAL (YAKHTEH), vol. 12, no. 2 (46), pp. 275–286, 2010, [Online]. Available: https://sid.ir/paper/16195/en

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