DEVELOPMENT AND APPLICATION OF REAL-TIME QUANTITATIVE POLYMERASE CHAIN REACTION TECHNIQUE USING SYBR GREEN I IN THE DIAGNOSIS OF DOWN SYNDROME

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KAMYAB AHMAD REZA; SHAHBAZI SHIRIN; DIBAJNIA P.; HAYAT NOUSAEID M.; AKBARI M.T.; RAFIEI ALI REZA; AKHLAGH TAMIZ K.; MAHDIAN R.

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Journal: Cell Journal (Yakhteh) Year:2010 | Volume:12 | Issue:2 (46) Page(s): 249-256

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Title

DEVELOPMENT AND APPLICATION OF REAL-TIME QUANTITATIVE POLYMERASE CHAIN REACTION TECHNIQUE USING SYBR GREEN I IN THE DIAGNOSIS OF DOWN SYNDROME

Author(s)

Keywords

DOWN SYNDROMEQ1

TRISOMY 21Q1

GENE COPY NUMBERQ1

REAL-TIMEQ1

PCRQ1

Abstract

Objective: Rapid diagnosis of TRISOMY 21 Syndrome (DOWN SYNDROME) patients using REAL-TIME quantitative Polymerase Chain Reaction (Real-Time qPCR) in order to establish a novel method for prenatal diagnosis in the future. Materials and Methods: A total of 5 ml of peripheral blood was obtained from each patient and normal controls (NR). Then, genomic DNA from lymphocytes was extracted using the salting out procedure. Gene dosage levels of DSCAM and (PMP22, DSCAM) in DOWN SYNDROME and NR were analyzed using REAL-TIME quantitative PCR. The DSCAM/PMP22 ratio was calculated according to the 2-DDCt formula for all samples.Results: REAL-TIME PCR showed a DSCAM/PMP22 ratio of 1.48±0.18 and 1.01±0.10 (p<0.001) in DOWN SYNDROME and normal samples, respectively, demonstrating three copies of the target (DSCAM) gene in TRISOMY 21 Syndrome.Conclusion: DSCAM/PMP22 ratio is increased significantly in DOWN SYNDROME patients than NR (1.5 times). Therefore, the REAL-TIME quantitative PCR technique can be used as a sensitive, accurate and reliable technique for rapid and prenatal diagnosis of TRISOMY 21 Syndrome.

Cites

INVESTIGATING THE EFFECT OF HYDROALCOHOLIC EXTRACT OF CYPERUS ROTUNDUS L. ON THE EXPRESSION OF BCL-X1 ANTIAPOPTOTIC GENE IN RATS’ HIPPOCAMPUS TISSUE FOLLOWING GLOBAL ISCHEMIC-REPERFUSION INJURY

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APA: Copy

KAMYAB, AHMAD REZA, SHAHBAZI, SHIRIN, DIBAJNIA, P., HAYAT NOUSAEID, M., AKBARI, M.T., RAFIEI, ALI REZA, AKHLAGH TAMIZ, K., & MAHDIAN, R.. (2010). DEVELOPMENT AND APPLICATION OF REAL-TIME QUANTITATIVE POLYMERASE CHAIN REACTION TECHNIQUE USING SYBR GREEN I IN THE DIAGNOSIS OF DOWN SYNDROME. CELL JOURNAL (YAKHTEH), 12(2 (46)), 249-256. SID. https://sid.ir/paper/16203/en

Vancouver: Copy

KAMYAB AHMAD REZA, SHAHBAZI SHIRIN, DIBAJNIA P., HAYAT NOUSAEID M., AKBARI M.T., RAFIEI ALI REZA, AKHLAGH TAMIZ K., MAHDIAN R.. DEVELOPMENT AND APPLICATION OF REAL-TIME QUANTITATIVE POLYMERASE CHAIN REACTION TECHNIQUE USING SYBR GREEN I IN THE DIAGNOSIS OF DOWN SYNDROME. CELL JOURNAL (YAKHTEH)[Internet]. 2010;12(2 (46)):249-256. Available from: https://sid.ir/paper/16203/en

IEEE: Copy

AHMAD REZA KAMYAB, SHIRIN SHAHBAZI, P. DIBAJNIA, M. HAYAT NOUSAEID, M.T. AKBARI, ALI REZA RAFIEI, K. AKHLAGH TAMIZ, and R. MAHDIAN, “DEVELOPMENT AND APPLICATION OF REAL-TIME QUANTITATIVE POLYMERASE CHAIN REACTION TECHNIQUE USING SYBR GREEN I IN THE DIAGNOSIS OF DOWN SYNDROME,” CELL JOURNAL (YAKHTEH), vol. 12, no. 2 (46), pp. 249–256, 2010, [Online]. Available: https://sid.ir/paper/16203/en

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