A COMPARISON BETWEEN THE COLONY FORMATION OF ADULT MOUSE SPERMATOGONIAL STEM CELLS IN CO CULTURES WITH SERTOLI AND STO (MOUSE EMBRYONIC FIBROBLAST CELL LINE)

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MOHAMMADI SEYEDEH MOUMENEH; MOVAHEDIN MANSOUREH; KOROUJI S.M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2010 | Volume:12 | Issue:2 (46) Page(s): 231-240

Download Full-Text

Persian Verion

View:

1,427

Download:

Cites:

Information Journal Paper

Title

A COMPARISON BETWEEN THE COLONY FORMATION OF ADULT MOUSE SPERMATOGONIAL STEM CELLS IN CO CULTURES WITH SERTOLI AND STO (MOUSE EMBRYONIC FIBROBLAST CELL LINE)

Author(s)

MOHAMMADI SEYEDEH MOUMENEH | MOVAHEDIN MANSOUREH | KOROUJI S.M. | Issue Writer Certificate

Keywords

ADULT SPERMATOGONIAL STEM CELLSQ1

COLONY FORMATIONQ1

CO CULTUREQ3

MOUSEQ2

Abstract

Objective: The aim of this study was to compare the COLONY FORMATION of spermatogonial stem cells (SSCs) on sertoli and STO (Mouse embryonic fibroblast cell line) feeder cell layers during a two-week period.Materials and Methods: Initially, sertoli cells and SSCs were isolated from adult MOUSE testes using a two-step enzymatic digestion and lectin immobilization. Characteristics of the isolated cells were immunocytochemically confirmed by examining for the presence of Oct-4, CDH1, promyelocytic leukaemia zinc finger factor (PLZF), SSC C-kit, and the distribution of Sertoli cell vimentin. SSCs were then cultured above the Sertoli, STO and the control (without co-culture) separately for two weeks. In all three groups, the number and diameter of colonies were evaluated using an invert microscope on the 3rd, 7th, 10th and 14th day. b1 and a6-integrin m-RNA expressions were assessed using a reverse transcription polymerase chain reaction (RT-PCR) and real-time PCR. Furthermore, Oct-4 m RNA expression was assessed using real time PCR. Statistical analysis was performed using ANOVA; and the paired two-sample t test and Tukey’s test were used as post-hoc tests for the data analysis of the three sertoli, STO and control cocultures.Results: At the four specified time points, our results showed significant differences (p<0.05) in colony numbers and diameters among the sertoli, STO and control groups. The number and diameter of colonies increased more rapidly in the sertoli coculture than in the other two Our results at all four time points also showed significant differences (p<0.05) in the mean colony numbers and diameters between the three groups, with the Sertoli coculture having the highest mean values for colony numbers and diameters. The RT-PCR results, after two-weeks of culturing, showed that b1-integrin was expressed in all three groups cocultures, but a6-integrin was not expressed. Additionally, based on real time PCR results, the three genes (b1-integrin, a6-integrin, Oct-4) mentioned were also expressed in all three CO CULTUREs groups.Conclusion: Based on the optimal effects of sertoli feeder cells on spermatogonial stem cells in a CO CULTURE system, as also confirmed by several other studies, their use is suggested to achieve better colonization of SSCs.

Cites

No record.

References

No record.

Cite

APA: Copy

MOHAMMADI, SEYEDEH MOUMENEH, MOVAHEDIN, MANSOUREH, & KOROUJI, S.M.. (2010). A COMPARISON BETWEEN THE COLONY FORMATION OF ADULT MOUSE SPERMATOGONIAL STEM CELLS IN CO CULTURES WITH SERTOLI AND STO (MOUSE EMBRYONIC FIBROBLAST CELL LINE). CELL JOURNAL (YAKHTEH), 12(2 (46)), 231-240. SID. https://sid.ir/paper/16208/en

Vancouver: Copy

MOHAMMADI SEYEDEH MOUMENEH, MOVAHEDIN MANSOUREH, KOROUJI S.M.. A COMPARISON BETWEEN THE COLONY FORMATION OF ADULT MOUSE SPERMATOGONIAL STEM CELLS IN CO CULTURES WITH SERTOLI AND STO (MOUSE EMBRYONIC FIBROBLAST CELL LINE). CELL JOURNAL (YAKHTEH)[Internet]. 2010;12(2 (46)):231-240. Available from: https://sid.ir/paper/16208/en

IEEE: Copy

SEYEDEH MOUMENEH MOHAMMADI, MANSOUREH MOVAHEDIN, and S.M. KOROUJI, “A COMPARISON BETWEEN THE COLONY FORMATION OF ADULT MOUSE SPERMATOGONIAL STEM CELLS IN CO CULTURES WITH SERTOLI AND STO (MOUSE EMBRYONIC FIBROBLAST CELL LINE),” CELL JOURNAL (YAKHTEH), vol. 12, no. 2 (46), pp. 231–240, 2010, [Online]. Available: https://sid.ir/paper/16208/en

Related Journal Papers