A COMPARISON OF SPERM ACROSOMAL REACTION ABILITY BY DOUBLE, TRIPLE, AND QUINACRINE STAINING METHODS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ANVARI M.; KALANTAR S.M.; NASRESFAHANI MOHAMMAD HOSSEIN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2003 | Volume:5 | Issue:17 Page(s): 49-55

Download Full-Text

Persian Verion

View:

1,179

Download:

Cites:

Information Journal Paper

Title

A COMPARISON OF SPERM ACROSOMAL REACTION ABILITY BY DOUBLE, TRIPLE, AND QUINACRINE STAINING METHODS

Author(s)

ANVARI M. | KALANTAR S.M. | NASRESFAHANI MOHAMMAD HOSSEIN | Issue Writer Certificate

Keywords

ACROSOME REACTIONQ3

DOUBLE STAININGQ3

TRIPLE STAININGQ3

QUINACRINEQ3

METHODSQ3

Abstract

Introduction: The acrosome is a sac shaped organelle contaning lysosomal enzyme that is located on the anterior of sperm head. During the ACROSOME REACTION its content is released in the vicinity of the egg. The acrosome reacted sperm then penetrates through the egg barriers. There are several METHODS for evaluating the acrosomal status; some of those are complex, expensive and/or non-accurate METHODS. The aim of this study was comparison between three of these METHODS, QUINACRINE fluorescent and current histochemical double & TRIPLE STAINING METHODS. Material and METHODS: Ten norm al ejaculate semen samples were selected according to the WHO, 1999 criteria. The motile sperm were liberated from seminal plasma using percoll preparation. The ACROSOME REACTION was then evaluated before and after capacitation, following induction by calcium ionophore for 1 to 2 hrs using three different QUINACRINE, double and TRIPLE STAINING METHODS. Results: There was no significant difference between the three different staining METHODS (P> 0.05), while after capacitation and induction using calcium ionophore the ACROSOME REACTION increased significantly (P< 0.01). In all experiments, the percentage of ACROSOME REACTION detected in quinacrine was significantly higher than the other METHODS (P< 0.01). There was a direct correlation among different METHODS (P< 0.001, r=7). Conclusion: The ACROSOME REACTION occurred in sperm following capacitation and induction by calcium ionophore. The QUINACRINE method is a rapid and simple method for detecting the acrosomal status of human spermatozoa.

Cites

No record.

References

Cite

APA: Copy

ANVARI, M., KALANTAR, S.M., & NASRESFAHANI, MOHAMMAD HOSSEIN. (2003). A COMPARISON OF SPERM ACROSOMAL REACTION ABILITY BY DOUBLE, TRIPLE, AND QUINACRINE STAINING METHODS . CELL JOURNAL (YAKHTEH), 5(17), 49-55. SID. https://sid.ir/paper/16213/en

Vancouver: Copy

ANVARI M., KALANTAR S.M., NASRESFAHANI MOHAMMAD HOSSEIN. A COMPARISON OF SPERM ACROSOMAL REACTION ABILITY BY DOUBLE, TRIPLE, AND QUINACRINE STAINING METHODS . CELL JOURNAL (YAKHTEH)[Internet]. 2003;5(17):49-55. Available from: https://sid.ir/paper/16213/en

IEEE: Copy

M. ANVARI, S.M. KALANTAR, and MOHAMMAD HOSSEIN NASRESFAHANI, “A COMPARISON OF SPERM ACROSOMAL REACTION ABILITY BY DOUBLE, TRIPLE, AND QUINACRINE STAINING METHODS ,” CELL JOURNAL (YAKHTEH), vol. 5, no. 17, pp. 49–55, 2003, [Online]. Available: https://sid.ir/paper/16213/en

Related Journal Papers