OPTIMIZATION OF PTS2-EGFP EXPRESSION IN CHO AND VERO CELLS

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GHODRATNAMA R.; KARBALAEI KH.; GHAEDI K.; BAHARVAND HOSSEIN; NASRESFAHANI MOHAMMAD HOSSEIN

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Journal: Cell Journal (Yakhteh) Year:2007 | Volume:9 | Issue:3 (35) Page(s): 170-175

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Title

OPTIMIZATION OF PTS2-EGFP EXPRESSION IN CHO AND VERO CELLS

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Keywords

EXPRESSION VECTORQ2

TARGETING SIGNALQ2

LIPOFECTIONQ2

LIPOPLEXQ2

Abstract

Objective: Reporter gene transfer to mammalian cells receives a great deal of attention due to its importance for molecular biology, embryology and developmental biology studies. Among DNA transfer technologies to eukaryotic cells, LIPOFECTION is known as the most widely used because of its easy handling procedure, low cell mortality and the natural pathway it undertakes.Materials and Methods: In this study we have examined the transfectability of two cell types: CHO and Vero cells via LIPOFECTION in four different treatments, with combination of exposure duration, 3 and 6 hrs, and different plasmid DNA concentration, 0.5 and 1mgs. A fusion protein EXPRESSION VECTOR, pUcD2. PTS2-EGFP was used to direct the EGFP protein to peroxisomes after expression of related cDNA. An SPSS analysis was preformed after counting the positive cells.Results: optimum gene expression was found when using 1mg DNA treated for three hrs for CHO cells, and 1mg DNA treated for six hrs for Vero cells.Conclusion: The result suggests that CHO LIPOFECTION efficiency is significantly increased by both the DNA concentration and exposure time increment; however, an increase in exposure time has less significant effect on low DNA concentration conditions. The same results have been observed for Vero cells. Optimum expression was obtained with highest DNA concentration.

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APA: Copy

GHODRATNAMA, R., KARBALAEI, KH., GHAEDI, K., BAHARVAND, HOSSEIN, & NASRESFAHANI, MOHAMMAD HOSSEIN. (2007). OPTIMIZATION OF PTS2-EGFP EXPRESSION IN CHO AND VERO CELLS. CELL JOURNAL (YAKHTEH), 9(3 (35)), 170-175. SID. https://sid.ir/paper/16244/en

Vancouver: Copy

GHODRATNAMA R., KARBALAEI KH., GHAEDI K., BAHARVAND HOSSEIN, NASRESFAHANI MOHAMMAD HOSSEIN. OPTIMIZATION OF PTS2-EGFP EXPRESSION IN CHO AND VERO CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2007;9(3 (35)):170-175. Available from: https://sid.ir/paper/16244/en

IEEE: Copy

R. GHODRATNAMA, KH. KARBALAEI, K. GHAEDI, HOSSEIN BAHARVAND, and MOHAMMAD HOSSEIN NASRESFAHANI, “OPTIMIZATION OF PTS2-EGFP EXPRESSION IN CHO AND VERO CELLS,” CELL JOURNAL (YAKHTEH), vol. 9, no. 3 (35), pp. 170–175, 2007, [Online]. Available: https://sid.ir/paper/16244/en

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