مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

952
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

281
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

2

Information Journal Paper

Title

EFFECT OF ASTROCYTE-CONDITIONED MEDIUM, RETINOIC ACID AND BASIC FIBROBLAST GROWTH FACTOR ON NEURAL DIFFERENTIATIN OF MOUSE EMBRYONIC STEM CELLS

Pages

  176-183

Abstract

 Objective: Embryonic stem cells (ESCs) are pluripotent cells capable of extensive proliferation while maintaining their potential to differentiate into any cell type. The therapeutic potential of these cells are promising, but in many cases limited by our inability to promote their differentiation. In this study we examined the effect of inducer factors in direct differentiation of mouse ESCs into a neural fate in suspension culture systems.Materials and Methods: Mouse ESCs (Royan B1) were cultivated in suspension to form embryoid bodies (EBs) within 2 days (2d). They were induced using ASTROCYTE-CONDITIONED MEDIUM (ACM), RETINOIC ACID (RA, 1mM) and BASIC FIBROBLAST GROWTH FACTOR (bFGF, 20 ng/ml) for 4 days (2+4d) and were cultured on poly-L-lysine coated dishes up to 5 days (2+4+5d) to differentiate. The expressions of neural specific genes were analyzed by immunocytochemistry and reverse transcription-polymerase chain reaction (RT-PCR). Differences of means for percentage significance of differentiated EBs were tested by the Mann-Whitney Test and for length and thickness of neural processes by t-test.Results: RETINOIC ACID increased the percentage of EBs outgrowth with neural morphology and bFGF had synergistic effect (at least p<0.05), while ACM did not influence NEURAL DIFFERENTIATION at all. Immunofluorescence analysis revealed the expression of b-tubulin III in neural cells and that the differentiated neural cells in RA+bFGF group were longer and thicker processes (at least p<0.05). The cells expressed Nestin, Pax6, NF-M, Islet-1, Lim1, and HB9.Conclusion: The results showed that ACM had no effect on NEURAL DIFFERENTIATION from ESCs, however, RA was in favor of this and that bFGF increased NEURAL DIFFERENTIATION synergistically by mechanisms that remain to be defined.

Cites

References

  • No record.

    • Cite

    APA: Copy

    MOGHADAS ALI, REZA, ZEYNALI, B., SOLEYMANI, MASOUD, HATAMI, MARYAM, & BAHARVAND, HOSSEIN. (2007). EFFECT OF ASTROCYTE-CONDITIONED MEDIUM, RETINOIC ACID AND BASIC FIBROBLAST GROWTH FACTOR ON NEURAL DIFFERENTIATIN OF MOUSE EMBRYONIC STEM CELLS. CELL JOURNAL (YAKHTEH), 9(3 (35)), 176-183. SID. https://sid.ir/paper/16245/en

    Vancouver: Copy

    MOGHADAS ALI REZA, ZEYNALI B., SOLEYMANI MASOUD, HATAMI MARYAM, BAHARVAND HOSSEIN. EFFECT OF ASTROCYTE-CONDITIONED MEDIUM, RETINOIC ACID AND BASIC FIBROBLAST GROWTH FACTOR ON NEURAL DIFFERENTIATIN OF MOUSE EMBRYONIC STEM CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2007;9(3 (35)):176-183. Available from: https://sid.ir/paper/16245/en

    IEEE: Copy

    REZA MOGHADAS ALI, B. ZEYNALI, MASOUD SOLEYMANI, MARYAM HATAMI, and HOSSEIN BAHARVAND, “EFFECT OF ASTROCYTE-CONDITIONED MEDIUM, RETINOIC ACID AND BASIC FIBROBLAST GROWTH FACTOR ON NEURAL DIFFERENTIATIN OF MOUSE EMBRYONIC STEM CELLS,” CELL JOURNAL (YAKHTEH), vol. 9, no. 3 (35), pp. 176–183, 2007, [Online]. Available: https://sid.ir/paper/16245/en

    Related Journal Papers

    Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top