DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO DORSAL INTERNEURONS OF THE SPINAL CORD USING BMP4 AND ACTIVIN A

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

NAJAFI FARZANEH; ZARE MEHRJERDI N.; HATAMI MARYAM; BAHARVAND HOSSEIN

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2009 | Volume:11 | Issue:3 (43) Page(s): 277-284

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

834

Download:

435

Cites:

Information Journal Paper

Title

DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO DORSAL INTERNEURONS OF THE SPINAL CORD USING BMP4 AND ACTIVIN A

Author(s)

NAJAFI FARZANEH | ZARE MEHRJERDI N. | HATAMI MARYAM | BAHARVAND HOSSEIN | Issue Writer Certificate

Keywords

EMBRYONIC STEM CELLSQ2

DORSAL PATTERNINGQ1

BMPQ1

ACTIVINQ1

SPINAL CORD INTERNEURONSQ1

Abstract

Objective: In vertebrates, bone morphogenetic proteins (BMPs) and ACTIVIN signals play multiple roles in dorso-ventral patterning and development of the spinal cord. Here the inductions of BMP 4 and ACTIVIN A on EMBRYONIC STEM CELLS (ESCs) into dorsal interneurons have been studied.Materials and Methods: Four different treatments have been used for mESC derived neural precursors; they include BMP4 (1ng/ml and 10ng/ml), ACTIVIN A (100ng/ml), and ACTIVIN A+BMP4 (100ng/ml, 10ng/ml). Induction’s effect on expression of specific dorsal interneuron markers in mature neurons have been evaluated by the use of immunocytochemistry and RT-PCR.Results: Treatment of ESC-derived neural precursors with BMP4, ACTIVIN A, or both showed an increased generation of both dI1 and dI3 interneurons (Lhx2 and Isl-1-positive cells) compared to the control group. However, the synergistic effect in generation of dI3 was not observed when both factors were used. Moreover, RT-PCR analysis of differentiated cells showed the expression of Lhx9, Lhx1, and Isl1, the transcription factors that are markers of dI1, dI2, and dI3 interneurons respectively.Conclusion: Our results showed that specific combination of developmental signaling molecules can direct the differentiation of ESCs into dorsal interneurons. Furthermore, qualitative and quantitative differences in signaling by different members of the TGF-β superfamily may play a role in the specification of different types of dorsal interneurons.

Cites

No record.

References

Cite

APA: Copy

NAJAFI, FARZANEH, ZARE MEHRJERDI, N., HATAMI, MARYAM, & BAHARVAND, HOSSEIN. (2009). DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO DORSAL INTERNEURONS OF THE SPINAL CORD USING BMP4 AND ACTIVIN A. CELL JOURNAL (YAKHTEH), 11(3 (43)), 277-284. SID. https://sid.ir/paper/16257/en

Vancouver: Copy

NAJAFI FARZANEH, ZARE MEHRJERDI N., HATAMI MARYAM, BAHARVAND HOSSEIN. DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO DORSAL INTERNEURONS OF THE SPINAL CORD USING BMP4 AND ACTIVIN A. CELL JOURNAL (YAKHTEH)[Internet]. 2009;11(3 (43)):277-284. Available from: https://sid.ir/paper/16257/en

IEEE: Copy

FARZANEH NAJAFI, N. ZARE MEHRJERDI, MARYAM HATAMI, and HOSSEIN BAHARVAND, “DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO DORSAL INTERNEURONS OF THE SPINAL CORD USING BMP4 AND ACTIVIN A,” CELL JOURNAL (YAKHTEH), vol. 11, no. 3 (43), pp. 277–284, 2009, [Online]. Available: https://sid.ir/paper/16257/en

Related Journal Papers