DESIGN OF MULTIPLEX POLYMERASE CHAIN REACTION (PCR) METHOD FOR MOLECULAR DETECTION OF YERSINIA PESTIS BACTERIUM

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

SOLEYMANI MOHAMMAD; EYNI F.; FALAH RAOUFI M.; NAZARI FIROUZEH; FARZAMPOUR SH.; JAMSHIDIAN E.; KHOUSHDEL A.R.; MAJIDZADEH K.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Cell Journal (Yakhteh) Year:2010 | Volume:12 | Issue:3 (47) Page(s): 363-370

Download Full-Text

Persian Verion

View:

916

Download:

Cites:

Information Journal Paper

Title

DESIGN OF MULTIPLEX POLYMERASE CHAIN REACTION (PCR) METHOD FOR MOLECULAR DETECTION OF YERSINIA PESTIS BACTERIUM

Author(s)

Keywords

YERSINIA PESTISQ1

DIAGNOSISQ1

PCRQ1

Abstract

Objective: YERSINIA PESTIS, the causative agent of the zoonotic plague infection, is a major public health concern both as a threat and potential bioweapon. The objective of the present study was to establish a uniplex and multiplex - polymerase chain reaction (PCR) test for the specific detection of Y. pestis.Materials and Methods: PCR reactions performed by three pair primers which targeted the caf1 and pla genes located on the pFra and pPst plasmids and the irp2 chromosomal gene located on the ‘pathogenicity island’. After TA cloning of the PCR products, the test’s limit of detection (LOD) was determined. For evaluating the specificity, PCR reactions were performed with negative control bacteria.Results: Assays were performed with the genome of Y. pestis which produced three DNA fragments of the expected sizes 300, 400 and 520 bp which corresponded to the irp2, caf1 and pla genes, respectively. The lower LoD was 370 copy numbers for the caf1 gene and 21 for the pla gene. In PCR reactions that used negative control bacteria, detectable fragments were not observed.Conclusion: Our method clearly discriminated Y. pestis DNA. The rapidity, specificity and sensitivity of this procedure suggest that it can serve as a useful alternative method for the inoculation of laboratory animals or the use of specific culture media for routine plaque surveillance and outbreak investigations. Another vital result of this study was the establishment of Y. pestis molecular detection technique in Iran.

Cites

No record.

References

No record.

Cite

APA: Copy

SOLEYMANI, MOHAMMAD, EYNI, F., FALAH RAOUFI, M., NAZARI, FIROUZEH, FARZAMPOUR, SH., JAMSHIDIAN, E., KHOUSHDEL, A.R., & MAJIDZADEH, K.. (2010). DESIGN OF MULTIPLEX POLYMERASE CHAIN REACTION (PCR) METHOD FOR MOLECULAR DETECTION OF YERSINIA PESTIS BACTERIUM. CELL JOURNAL (YAKHTEH), 12(3 (47)), 363-370. SID. https://sid.ir/paper/16307/en

Vancouver: Copy

SOLEYMANI MOHAMMAD, EYNI F., FALAH RAOUFI M., NAZARI FIROUZEH, FARZAMPOUR SH., JAMSHIDIAN E., KHOUSHDEL A.R., MAJIDZADEH K.. DESIGN OF MULTIPLEX POLYMERASE CHAIN REACTION (PCR) METHOD FOR MOLECULAR DETECTION OF YERSINIA PESTIS BACTERIUM. CELL JOURNAL (YAKHTEH)[Internet]. 2010;12(3 (47)):363-370. Available from: https://sid.ir/paper/16307/en

IEEE: Copy

MOHAMMAD SOLEYMANI, F. EYNI, M. FALAH RAOUFI, FIROUZEH NAZARI, SH. FARZAMPOUR, E. JAMSHIDIAN, A.R. KHOUSHDEL, and K. MAJIDZADEH, “DESIGN OF MULTIPLEX POLYMERASE CHAIN REACTION (PCR) METHOD FOR MOLECULAR DETECTION OF YERSINIA PESTIS BACTERIUM,” CELL JOURNAL (YAKHTEH), vol. 12, no. 3 (47), pp. 363–370, 2010, [Online]. Available: https://sid.ir/paper/16307/en

Related Journal Papers