EFFECT OF EXTRACELLULAR MATRIX ON DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO INSULIN-PRODUCING CELLS

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FAROKHI ALI; ELAHI E.; PIROUZ MAHDI; JAFARI HANIEH; BAHARVAND HOSSEIN

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Journal: Cell Journal (Yakhteh) Year:2009 | Volume:10 | Issue:4 (40) Page(s): 250-259

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Title

EFFECT OF EXTRACELLULAR MATRIX ON DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO INSULIN-PRODUCING CELLS

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EMBRYONIC STEM CELLQ1

DIFFERENTIATIONQ2

β -CELLQ2

EXTRACELLULAR MATRIXQ1

Abstract

Objective: Evaluation of extracellular matrics (ECMs) effect on DIFFERENTIATION of EMBRYONIC STEM CELLs (ESCs) to pancreatic β-cell.Materials and Methods: Mouse ESC line, Royan B1, was subjected to DIFFERENTIATION into b -like cells in a three-step method: generation of embryoid bodies (EBs), spontaneous DIFFERENTIATION and induction by Nicotinamide onto different matrices including poly L-ornithine/laminin, gelatin, and two different dilution of matrigel (1:30, 1:100) and control group (no ECM). At the final step, differentiated cells were analyzed for expression of some pancreas-specific genes using "semi-quantitative RT-PCR ", for detection of insulin and C-peptide presence in cells using "immunocytochemistry" and for the evaluation of the amount of secreted insulin in response to glucose Using "insulin secretion assay".Results: The semi-quantitative RT-PCR analysis of differentiated cells on 1:30 matrigel coated-plates showed consistent higher expression of b-cell specific markers including Insulin I, Insulin II, Slc2a2 in comparison to the other ECMs. The results of immunostainig for C-peptide showed no significant differences between the experimental groups and finally insulin secretion assay revealed that differentiated cells on 1:30 matrigel coated-plates secreted more insulin in response to glucose in comparison to the other ECMs.Conclusion: Our results suggest that type of ECM may influence ESC DIFFERENTIATION into insulin-secreting cells and 1:30 matrigel was more effective. However, the success rate of DIFFERENTIATION needs further investigations using other appropriate ECMs.

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APA: Copy

FAROKHI, ALI, ELAHI, E., PIROUZ, MAHDI, JAFARI, HANIEH, & BAHARVAND, HOSSEIN. (2009). EFFECT OF EXTRACELLULAR MATRIX ON DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO INSULIN-PRODUCING CELLS. CELL JOURNAL (YAKHTEH), 10(4 (40)), 250-259. SID. https://sid.ir/paper/16366/en

Vancouver: Copy

FAROKHI ALI, ELAHI E., PIROUZ MAHDI, JAFARI HANIEH, BAHARVAND HOSSEIN. EFFECT OF EXTRACELLULAR MATRIX ON DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO INSULIN-PRODUCING CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2009;10(4 (40)):250-259. Available from: https://sid.ir/paper/16366/en

IEEE: Copy

ALI FAROKHI, E. ELAHI, MAHDI PIROUZ, HANIEH JAFARI, and HOSSEIN BAHARVAND, “EFFECT OF EXTRACELLULAR MATRIX ON DIFFERENTIATION OF MOUSE EMBRYONIC STEM CELLS INTO INSULIN-PRODUCING CELLS,” CELL JOURNAL (YAKHTEH), vol. 10, no. 4 (40), pp. 250–259, 2009, [Online]. Available: https://sid.ir/paper/16366/en

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