MOLECULAR IDENTIFICATION OF MYCOBACTERIUM PARATUBERCULOSIS ISOLATED FROM DAIRY CATTLES IN CITY TONEKABON (NORTHERN IRAN)

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SADATI R.; JAFARPOUR M.; NAZEMI A.; BARGHI A.

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Journal: JOURNAL OF BIOLOGY SCIENCE Year:2010 | Volume:3 | Issue:4 (SERIES NO. 11) Page(s): 51-58

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Information Journal Paper

Title

MOLECULAR IDENTIFICATION OF MYCOBACTERIUM PARATUBERCULOSIS ISOLATED FROM DAIRY CATTLES IN CITY TONEKABON (NORTHERN IRAN)

Author(s)

SADATI R. | JAFARPOUR M. | NAZEMI A. | BARGHI A. | Issue Writer Certificate

Keywords

IRANQ2

TONEKABONQ3

MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSISQ3

JOHNE'S DISEASEQ3

DAIRY CATTLESQ3

Abstract

Recent study is the first attempt to identify infected dairy cattle with Mycobacterium paratuberculosis which is done with John in test and PCR method in TONEKABON.Paratuberculosis is a chronic intestinal infection in ruminants and spread in different ways to environment. Milk is important source of bacteria. Also, due to the possible relationship between JOHNE'S DISEASE and Crohn's disease in humans, presence of bacteria in milk can be an important index to assess the spread of infection in animal and human. In this study, at first, 90 dairy cattle were tested by John in test and result were as follow, 3 positive cases, 23 suspected cases and 62 negative cases. Then the samples examined using nested PCR, then two pairs of primers were used to increase of desired pieces of 194 bp were used. The prevalence results were different in three verify regions and showed desired ranges between 4.2 and 7.7%. As a result, tests revealed that Johnin test doesn't have an ability to identify suspected cases, then it is necessary to use PCR techinique to identify the exact animal patient.

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APA: Copy

SADATI, R., JAFARPOUR, M., NAZEMI, A., & BARGHI, A.. (2010). MOLECULAR IDENTIFICATION OF MYCOBACTERIUM PARATUBERCULOSIS ISOLATED FROM DAIRY CATTLES IN CITY TONEKABON (NORTHERN IRAN). JOURNAL OF BIOLOGY SCIENCE, 3(4 (SERIES NO. 11)), 51-58. SID. https://sid.ir/paper/164127/en

Vancouver: Copy

SADATI R., JAFARPOUR M., NAZEMI A., BARGHI A.. MOLECULAR IDENTIFICATION OF MYCOBACTERIUM PARATUBERCULOSIS ISOLATED FROM DAIRY CATTLES IN CITY TONEKABON (NORTHERN IRAN). JOURNAL OF BIOLOGY SCIENCE[Internet]. 2010;3(4 (SERIES NO. 11)):51-58. Available from: https://sid.ir/paper/164127/en

IEEE: Copy

R. SADATI, M. JAFARPOUR, A. NAZEMI, and A. BARGHI, “MOLECULAR IDENTIFICATION OF MYCOBACTERIUM PARATUBERCULOSIS ISOLATED FROM DAIRY CATTLES IN CITY TONEKABON (NORTHERN IRAN),” JOURNAL OF BIOLOGY SCIENCE, vol. 3, no. 4 (SERIES NO. 11), pp. 51–58, 2010, [Online]. Available: https://sid.ir/paper/164127/en

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