THE STUDY OF GENETIC DIVERSITY BY PEANUT GENOTYPES BY THE METHOD OF ELECTROPHORESIS SDS-PAGE

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SADEGHI S.M.; JAVID F.; MOHAMADIAN ROSHAN N.

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Journal: JOURNAL OF BIOLOGY SCIENCE Year:2011 | Volume:5 | Issue:3 (SERIES NO. 18) Page(s): 87-95

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Title

THE STUDY OF GENETIC DIVERSITY BY PEANUT GENOTYPES BY THE METHOD OF ELECTROPHORESIS SDS-PAGE

Author(s)

SADEGHI S.M. | JAVID F. | MOHAMADIAN ROSHAN N. | Issue Writer Certificate

Keywords

ELECTROPHORESISQ3

PEANUTQ2

GENETIC DIVERSITYQ3

CLUSTER ANALYSISQ2

GENOTYPEQ2

Abstract

In order to study GENETIC DIVERSITY of PEANUT GENOTYPEs in both drought stress and normal moisture conditions by the molecular method by using ELECTROPHORESIS polyacrylamid gel ELECTROPHORESIS, an experiment was conducted in agriculture department of Islamic Azad University of Lahijan branch in 1390. In this experiment the diversity of GENOTYPEs on the basis storage protein of seed was studies. Among ELECTROPHORESIS methods 10 and 5 percent of concentration polyacrylamid successively for the main and basic gel together 50ml of concentration buffer, 5μl of sample quantity in the hole and two hour coloring for achieving the best resolution of bands was identified. The results ELECTROPHORESIS of the protein 8-12 bands on the gel which the bands with high molecular weight and best resolution for study was identified. CLUSTER ANALYSIS for base banding pattern, separated GENOTYPEs to three and four groups in drought and non-drought stress, respectively. In both conditions (drought and normal) 2 monomorphism and 6 polymorphism groups was observed.

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APA: Copy

SADEGHI, S.M., JAVID, F., & MOHAMADIAN ROSHAN, N.. (2011). THE STUDY OF GENETIC DIVERSITY BY PEANUT GENOTYPES BY THE METHOD OF ELECTROPHORESIS SDS-PAGE. JOURNAL OF BIOLOGY SCIENCE, 5(3 (SERIES NO. 18)), 87-95. SID. https://sid.ir/paper/164219/en

Vancouver: Copy

SADEGHI S.M., JAVID F., MOHAMADIAN ROSHAN N.. THE STUDY OF GENETIC DIVERSITY BY PEANUT GENOTYPES BY THE METHOD OF ELECTROPHORESIS SDS-PAGE. JOURNAL OF BIOLOGY SCIENCE[Internet]. 2011;5(3 (SERIES NO. 18)):87-95. Available from: https://sid.ir/paper/164219/en

IEEE: Copy

S.M. SADEGHI, F. JAVID, and N. MOHAMADIAN ROSHAN, “THE STUDY OF GENETIC DIVERSITY BY PEANUT GENOTYPES BY THE METHOD OF ELECTROPHORESIS SDS-PAGE,” JOURNAL OF BIOLOGY SCIENCE, vol. 5, no. 3 (SERIES NO. 18), pp. 87–95, 2011, [Online]. Available: https://sid.ir/paper/164219/en

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