A COMPARISON BETWEEN PCR AND BACTERIOLOGY METHOD IN DIAGNOSIS BACTERIAL MENINGITIS

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GOUDARZI H.; KAZEMI B.; YARAEI K.; NAVIDI M.; BANDEHPOUR M.

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Paper Information

Journal: Cell Journal (Yakhteh) Year:2002 | Volume:4 | Issue:14 Page(s): 101-104

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Title

A COMPARISON BETWEEN PCR AND BACTERIOLOGY METHOD IN DIAGNOSIS BACTERIAL MENINGITIS

Author(s)

Keywords

PCRQ3

16S RRNAQ3

MENIGITISQ3

LUMBAR PUNCTUREQ3

Abstract

Introduction: Meningitis is one of the lethal diseases and its prompt diagnosis is of utmost importance. Current diagnostic methods (eg. bacterial cultures, cellular cultures, biochemical methods and cell count in CSF) are not sensitive enough. Therefore dorelopnga new method for quick diagnosis is essential.Material and Methods: We extracted DNA from cerebrospinal fluid and PCR was performed by primers which amplified the 16S RRNA sequence. Microbial culture was also performed for comparison.Results: We examined 51 specimens, among which 23.5% were culture positive. By PCR method, we reported 41.1 positive cases. Therefore if PCR is not used, almost half of the positive cases will be missed.Conclusion: We concluded that PCR is the best method of diagnosis, with high accuracy and precision for detecting microorganisms in sterile specimens in 3-4 hours. Since the 16S RRNA sequence has been similar in all prokaryotes, this sequence is considered appropriate for target for PCR.

Cites

DETERMINATION OF BACTERIAL ETIOLOGY OF 100 CSF SAMPLES OF PATIENTS WITH MENINGITIS AT FOUR MILITARY HOSPITALS IN TEHRAN BETWEEN 2003 AND 2005

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APA: Copy

GOUDARZI, H., KAZEMI, B., YARAEI, K., NAVIDI, M., & BANDEHPOUR, M.. (2002). A COMPARISON BETWEEN PCR AND BACTERIOLOGY METHOD IN DIAGNOSIS BACTERIAL MENINGITIS. CELL JOURNAL (YAKHTEH), 4(14), 101-104. SID. https://sid.ir/paper/16422/en

Vancouver: Copy

GOUDARZI H., KAZEMI B., YARAEI K., NAVIDI M., BANDEHPOUR M.. A COMPARISON BETWEEN PCR AND BACTERIOLOGY METHOD IN DIAGNOSIS BACTERIAL MENINGITIS. CELL JOURNAL (YAKHTEH)[Internet]. 2002;4(14):101-104. Available from: https://sid.ir/paper/16422/en

IEEE: Copy

H. GOUDARZI, B. KAZEMI, K. YARAEI, M. NAVIDI, and M. BANDEHPOUR, “A COMPARISON BETWEEN PCR AND BACTERIOLOGY METHOD IN DIAGNOSIS BACTERIAL MENINGITIS,” CELL JOURNAL (YAKHTEH), vol. 4, no. 14, pp. 101–104, 2002, [Online]. Available: https://sid.ir/paper/16422/en

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