DETERMINATION OF PREDOMINANT GENOTYPE OF HELICOBACTER PYLORI IN PATIENTS WITH GASTRODUODENAL IN TONEKABON CITY

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DALIR KOUHI M.; ASMAR MAHDI; MOZAFARI N.A.

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Paper Information

Journal: JOURNAL OF BIOLOGY SCIENCE Year:2008 | Volume:2 | Issue:3 (SERIES NO. 6) Page(s): 11-21

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Title

DETERMINATION OF PREDOMINANT GENOTYPE OF HELICOBACTER PYLORI IN PATIENTS WITH GASTRODUODENAL IN TONEKABON CITY

Author(s)

DALIR KOUHI M. | ASMAR MAHDI | MOZAFARI N.A. | Issue Writer Certificate

Keywords

TONKABUONQ3

HELICOBACTER PYLORIQ3

CAG A GENEQ3

ICE A GENEQ3

PEPTIC ULCERQ3

Abstract

Helicobacter Pylori colonize in gastric mucosa and causes disorders in upper gastro intestine. The clinical outcome of HELICOBACTER PYLORI infection may be associated with or without Cag A or ICE A GENEs.To investigate presence of cag A and ICE A GENEs in HELICOBACTER PYLORI strains among patients with gastritis, Gastric ulcer and duodenal ulcer, 80 gastric and duodenal biopsy specimens were taken. Urease test was perfomed for all specimens that H.pylori strains were isolated from 25 patients. We Perfomed DNA extraction used allelic specific PCR to examine iceA and Cag A status of H.pylori isolates. H. pylori was isolated in 25 cases. The PCR method indicates prevalence of iceA gene in strains isolated from patients with gastritis, gastric ulcer and duodenal ulcer was 25%, 50% and 25% respectively. Additionally iceA1 gene was found in 75% of the isolates strains and it was predominant. Prevalence of cagA genes in strains isolated from patients with gastritis, Gastric ulcer and duodenal ulcer was 36/36%, 50% and 16/16% respectively. According to the finding it is suggested the prescense of cagA gene in strains of HELICOBACTER PYLORI may play an important role in severity disorders or PEPTIC ULCER. But in conclusion we cannot be suggested any of these gene tobe an determining the nature of clinical disease byH. pylori infection, Various geographiced region,may be associated with different frequency in cagA and iceA.

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References

A STUDY OF CAGA GENE IN HELICOBACTER PYLORI STRAINS ISOLATED FROM PATIENTS WITH NUD, PEPTIC ULCER AND GASTRIC CANCER BY PCR METHOD

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APA: Copy

DALIR KOUHI, M., ASMAR, MAHDI, & MOZAFARI, N.A.. (2008). DETERMINATION OF PREDOMINANT GENOTYPE OF HELICOBACTER PYLORI IN PATIENTS WITH GASTRODUODENAL IN TONEKABON CITY. JOURNAL OF BIOLOGY SCIENCE, 2(3 (SERIES NO. 6)), 11-21. SID. https://sid.ir/paper/164226/en

Vancouver: Copy

DALIR KOUHI M., ASMAR MAHDI, MOZAFARI N.A.. DETERMINATION OF PREDOMINANT GENOTYPE OF HELICOBACTER PYLORI IN PATIENTS WITH GASTRODUODENAL IN TONEKABON CITY. JOURNAL OF BIOLOGY SCIENCE[Internet]. 2008;2(3 (SERIES NO. 6)):11-21. Available from: https://sid.ir/paper/164226/en

IEEE: Copy

M. DALIR KOUHI, MAHDI ASMAR, and N.A. MOZAFARI, “DETERMINATION OF PREDOMINANT GENOTYPE OF HELICOBACTER PYLORI IN PATIENTS WITH GASTRODUODENAL IN TONEKABON CITY,” JOURNAL OF BIOLOGY SCIENCE, vol. 2, no. 3 (SERIES NO. 6), pp. 11–21, 2008, [Online]. Available: https://sid.ir/paper/164226/en

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