USING FLUORSCENCE IN SITU HYBRIDIZATION (FISH) TECHNIQUE FOR EVALUATION OF FREQUENCY OF CHROMOSOMAL REARRANGEMENTS IN ATAXIA TELANGIECTASIA LYMPHOBLASTOID CELLS

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Journal: Cell Journal (Yakhteh) Year:1999 | Volume:1 | Issue:1 Page(s): 39-48

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Title

USING FLUORSCENCE IN SITU HYBRIDIZATION (FISH) TECHNIQUE FOR EVALUATION OF FREQUENCY OF CHROMOSOMAL REARRANGEMENTS IN ATAXIA TELANGIECTASIA LYMPHOBLASTOID CELLS

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Keywords

FISH TECHNIQUEQ4

CHROMOSOMAL REARRANGEMENTSQ4

ATAXIA – TELANGIECTASIA CELLSQ4

Abstract

Introduction: Fluorscence in situ hybridization (FISH) enables specific detection of unique sequences of varying length, chromosomal regions or entire chromosomes within metaphase or interphase cells. Recent developments in this technology permit the rapid mapping and ordering of DNA fragments on single metaphase chromosome bands. The technique of FISH incorporates several stages including: probe preparation and labelling, hybridization of probe with chromosome preparation and detection of signal, and imaging. Using these technique chromosomal alterations in ataxia telangiectasia lymphoblastoid cells which are highly sensitive to clastogenic and mutagnic effects of chemnical and physical agents were investigated. Materials and Methods: Normal and A-T cells were grown in RPMI – 1640 medium and irradiated at G2 and G1 phases of the cell cycle. After democolcine treatment and metaphase chromosome preparation, slides were prepared and FISH is performed for all samples using whole chromosome probe for chromosomes 5, 1, 4, 7 and 14. Fifty mitoses were analysed for each sample by Zeiss fluorescent microscope attached to a computerized programme. Results: Analysis of normal and A – T mitoses before and after irradiation revealed a very low frequency of chromosomal abnormalities, for specific chromosomes 5 painted in this study. However, more aberration was found in A-T cells of varios CHROMOSOMAL REARRANGEMENTS such as translocation, trisomy, Robertsonian translocation and chromatid type breaks. Conclusion: In routin cytogentic methodologies visualisation of various CHROMOSOMAL REARRANGEMENTS is not always possible in a single preparation. Although detecton sensitivity of FISH is limited to the specific chromosomes, but as seen for A – T cells, FISH can be considered as one of the most effective method for visualization of various chromosomal alterations. This technique can have wide application not only in human genome mapping and the genome of other organisms, but also in clinical cytogenetics, somatic cell genetics, cancer diagnosis and gene expression studies.

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APA: Copy

. (1999). USING FLUORSCENCE IN SITU HYBRIDIZATION (FISH) TECHNIQUE FOR EVALUATION OF FREQUENCY OF CHROMOSOMAL REARRANGEMENTS IN ATAXIA TELANGIECTASIA LYMPHOBLASTOID CELLS. CELL JOURNAL (YAKHTEH), 1(1), 39-48. SID. https://sid.ir/paper/16446/en

Vancouver: Copy

. USING FLUORSCENCE IN SITU HYBRIDIZATION (FISH) TECHNIQUE FOR EVALUATION OF FREQUENCY OF CHROMOSOMAL REARRANGEMENTS IN ATAXIA TELANGIECTASIA LYMPHOBLASTOID CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 1999;1(1):39-48. Available from: https://sid.ir/paper/16446/en

IEEE: Copy

, “USING FLUORSCENCE IN SITU HYBRIDIZATION (FISH) TECHNIQUE FOR EVALUATION OF FREQUENCY OF CHROMOSOMAL REARRANGEMENTS IN ATAXIA TELANGIECTASIA LYMPHOBLASTOID CELLS,” CELL JOURNAL (YAKHTEH), vol. 1, no. 1, pp. 39–48, 1999, [Online]. Available: https://sid.ir/paper/16446/en

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