EFFECTS OF DIFFERENT DOSES OF BONE MORPHOGENETIC PROTEIN 4 ON VIABILITY AND PROLIFERATION RATES OF MOUSE EMBRYONIC STEM CELLS

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MAKOULATI Z.; MOVAHEDIN MANSOUREH; FOROUZANDEH MOGHADAM M.

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Journal: Cell Journal (Yakhteh) Year:2009 | Volume:11 | Issue:1 (41) Page(s): 29-34

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Title

EFFECTS OF DIFFERENT DOSES OF BONE MORPHOGENETIC PROTEIN 4 ON VIABILITY AND PROLIFERATION RATES OF MOUSE EMBRYONIC STEM CELLS

Author(s)

MAKOULATI Z. | MOVAHEDIN MANSOUREH | FOROUZANDEH MOGHADAM M. | Issue Writer Certificate

Keywords

EMBRYONIC STEM CELLSQ2

BMP4Q1

PROLIFERATIONQ1

VIABILITYQ3

Abstract

Objective: In this study, we examined the effect of different doses of bone morphogenetic protein 4 (BMP4) on CCE mouse EMBRYONIC STEM CELLS (ESCs) VIABILITY and PROLIFERATION rates in order to improve the outcome of induction processes and make a system with highest VIABILITY and PROLIFERATION rates for further studies on BMP4 roles in multiple developmental stages.Materials and Methods: Expression of Oct-4 was studied and confirmed in this cell line immunocytochemically. Also, in order to evaluate the PROLIFERATION and VIABILITY rates in BMP4-treated cells, ESCs were cultured in Dulbecco's Modified Eagle Medium (DMEM) containing different doses of BMP4 (0, 0.01, 0.1, 1, 5, 25, 50 and 100ng/ml). The mean number of whole cells and living cells were considered as PROLIFERATION and survival rates respectively. Data analysis was done with ANOVA test.Results: The results showed that there were significant differences between the mean percent of VIABILITY between 1ng/ml and 0 ng/ml (control) and 50 and 100 ng/ml BMP4 (p£0.01), as well as between 5 ng/ml and 0, 0.01, 0.1, 25, 50 and 100 ng/ml BMP4 (p£0.02). Also, significant differences were observed in PROLIFERATION rates between 5 ng/ml and 0, 0.01, 0.1, 1, 25 and 100 ng/ml BMP4 (p£0.01), 25 ng/ml and 0.01, 1 and 5 ng/ml BMP4 (p£0.01), as well as between 50 ng/ml and 0.01 and 0.1 ng/ml BMP4 (p£0.001).Conclusion: The results suggest that addition of 5ng/ml BMP4 had the best effects on the PROLIFERATION and VIABILITY rates of CCE mouse ESCs.

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APA: Copy

MAKOULATI, Z., MOVAHEDIN, MANSOUREH, & FOROUZANDEH MOGHADAM, M.. (2009). EFFECTS OF DIFFERENT DOSES OF BONE MORPHOGENETIC PROTEIN 4 ON VIABILITY AND PROLIFERATION RATES OF MOUSE EMBRYONIC STEM CELLS. CELL JOURNAL (YAKHTEH), 11(1 (41)), 29-34. SID. https://sid.ir/paper/16525/en

Vancouver: Copy

MAKOULATI Z., MOVAHEDIN MANSOUREH, FOROUZANDEH MOGHADAM M.. EFFECTS OF DIFFERENT DOSES OF BONE MORPHOGENETIC PROTEIN 4 ON VIABILITY AND PROLIFERATION RATES OF MOUSE EMBRYONIC STEM CELLS. CELL JOURNAL (YAKHTEH)[Internet]. 2009;11(1 (41)):29-34. Available from: https://sid.ir/paper/16525/en

IEEE: Copy

Z. MAKOULATI, MANSOUREH MOVAHEDIN, and M. FOROUZANDEH MOGHADAM, “EFFECTS OF DIFFERENT DOSES OF BONE MORPHOGENETIC PROTEIN 4 ON VIABILITY AND PROLIFERATION RATES OF MOUSE EMBRYONIC STEM CELLS,” CELL JOURNAL (YAKHTEH), vol. 11, no. 1 (41), pp. 29–34, 2009, [Online]. Available: https://sid.ir/paper/16525/en

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