IDENTIFICATION OF CD4+ T-CELL EPITOPES ON MYCOBACTERIUM TUBERCULOSIS- SECRETED MPB51 PROTEIN IN C57BL/6 MICE

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RAFIEI ALI REZA; KUADE Y.

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Journal: Avicenna Journal of Clinical Medicine Year:2006 | Volume:12 | Issue:4 (SN 38) Page(s): 28-35

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Title

IDENTIFICATION OF CD4+ T-CELL EPITOPES ON MYCOBACTERIUM TUBERCULOSIS- SECRETED MPB51 PROTEIN IN C57BL/6 MICE

Author(s)

RAFIEI ALI REZA | KUADE Y. | Issue Writer Certificate

Keywords

EPITOPE MAPPINGQ3

EPITOPEQ3

T-CELLQ3

MICEQ3

TUBERCULOSISQ3

Abstract

Introduction & Objective: Both CD4+ type 1 helper (Th1) cells and CD8+T cells play effective roles in protection against Mycobacterium TUBERCULOSIS infection. DNA vaccine encoding MPB51 can induce Th1-type immune responses and protective immunity upon challenge with M.tuberculosis. This study address to identify T-CELL immunodominant EPITOPEs on MPB51 in C57BL/6mice.Materials & Methods: We cloned DNA encoding MPB51 molecule in pCI plasmid. After constructing MPB51 DNA-covered gold cartridge, C57BL/6 MICE were immunized by using a gene gun system. Two weeks after the last immunization, the immune spleen cells were cultured in the presence of a synthetic overlapping library peptides covering the mature MPB51 sequence or medium alone. Intracellular and cell culture supernatant gamma interferon (IFN-γ) production was analyzed using flow cytometry and ELISA, respectively. Results: Mapping of T-CELL EPITOPEs on MPB51 molecule was performed in the spleen lymphocytes restimulated by 20-mer overlapping synthetic peptides of mature MPB51 sequence. Flow cytometric analysis with intracellular IFN-g and the T-CELL phenotype revealed that P171-190 and P191-210 peptides contain immunodominant CD4+T-cell EPITOPEs. Further analysis by using T-CELL subset depletion and serial peptide dilution revealed that P171 and p191 are H2-Ab-restricteddominantand subdominant CD4+T cell EPITOPEs, respectively.Conclusion: This study proved that vaccination with plasmid DNA encoding M. TUBERCULOSIS-secreted MPB51 protein not only induce CD4+T cells immune response but also is an appropriate method for identifying immunogenic peptides.

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APA: Copy

RAFIEI, ALI REZA, & KUADE, Y.. (2006). IDENTIFICATION OF CD4⁺ T-CELL EPITOPES ON MYCOBACTERIUM TUBERCULOSIS- SECRETED MPB51 PROTEIN IN C57BL/6 MICE. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), 12(4 (SN 38)), 28-35. SID. https://sid.ir/paper/17862/en

Vancouver: Copy

RAFIEI ALI REZA, KUADE Y.. IDENTIFICATION OF CD4⁺ T-CELL EPITOPES ON MYCOBACTERIUM TUBERCULOSIS- SECRETED MPB51 PROTEIN IN C57BL/6 MICE. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES)[Internet]. 2006;12(4 (SN 38)):28-35. Available from: https://sid.ir/paper/17862/en

IEEE: Copy

ALI REZA RAFIEI, and Y. KUADE, “IDENTIFICATION OF CD4⁺ T-CELL EPITOPES ON MYCOBACTERIUM TUBERCULOSIS- SECRETED MPB51 PROTEIN IN C57BL/6 MICE,” AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), vol. 12, no. 4 (SN 38), pp. 28–35, 2006, [Online]. Available: https://sid.ir/paper/17862/en

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