DETECTION OF RESISTANT GENE TO METHICILLIN (MEC-A) IN PURULENT INFECTIONS RELATED TO STAPHYLOCOCCUS USING PCR

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SADEGHIAN S.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Avicenna Journal of Clinical Medicine Year:2001 | Volume:8 | Issue:2 (SN 20) Page(s): 13-18

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Title

DETECTION OF RESISTANT GENE TO METHICILLIN (MEC-A) IN PURULENT INFECTIONS RELATED TO STAPHYLOCOCCUS USING PCR

Author(s)

SADEGHIAN S. | Issue Writer Certificate

Keywords

GENEQ4

MEC-AQ4

METHICILLINQ3

POLYMERASE CHAIN REACTIONQ2

STAPHYLOCOCCAL INFECTIONSQ2

Abstract

The emergence of Staphylococcus strains resistant to METHICILLIN occurred shortly after the introduction of METHICILLIN into clinical use. Resistance to METHICILLIN in clinical isolates of Staphylococci has been explained by the production of a characteristic penicillin-binding protein (PBP), designated PBP2a, that has a decreased binding affinity for - lactam antibiotics, the GENE mec-A encoding PBP2a was cloned and is distributed among METHICILLIN- resistant but not METHICILLIN-susceptible populations of S. aureus. Recently, high prevalence of METHICILLIN resistance has been noticed in the clinical isolates of coagulase-negative Staphylococci (CNS) which, besides MRSA (Methicillin- Resistant -Staphylococcus aureus), also have been recognized as important nosocomial pathogens. Detection of the MEC-A using PCR may provide a sensitive method for identifying METHICILLIN resistant Staphylococci. In this study, overall 268 wound swabs in which 107 cases (40%) were Staphylococcus aureus and 10 cases (3.7%) were coagulase negative Staphylococcus and the rest of them were other microorganisms were examined . After the extraction of DNA, PCR was carried out using specific primers. The results indicated that the MEC-A was present in 24 cases (9%). In 8 cases (34%) Staphylococcus aureus,3 cases (12%) coagulase negative Staphylococci, the others were related to another microorgamisms that probably were contaminated by Staphylococcus species.

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APA: Copy

SADEGHIAN, S.. (2001). DETECTION OF RESISTANT GENE TO METHICILLIN (MEC-A) IN PURULENT INFECTIONS RELATED TO STAPHYLOCOCCUS USING PCR . AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), 8(2 (SN 20)), 13-18. SID. https://sid.ir/paper/17894/en

Vancouver: Copy

SADEGHIAN S.. DETECTION OF RESISTANT GENE TO METHICILLIN (MEC-A) IN PURULENT INFECTIONS RELATED TO STAPHYLOCOCCUS USING PCR . AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES)[Internet]. 2001;8(2 (SN 20)):13-18. Available from: https://sid.ir/paper/17894/en

IEEE: Copy

S. SADEGHIAN, “DETECTION OF RESISTANT GENE TO METHICILLIN (MEC-A) IN PURULENT INFECTIONS RELATED TO STAPHYLOCOCCUS USING PCR ,” AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), vol. 8, no. 2 (SN 20), pp. 13–18, 2001, [Online]. Available: https://sid.ir/paper/17894/en

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