MOLECULAR DETECTION OF VIRULENCE GENES AMONG PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM CLINICAL SPECIMENS BY MULTIPLEX PCR

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SHARIAT AFSOON; ALIZADEH SAJAD; JAHANGIRI HOSEINABADI MEHDI; MOVAGHARNEJAD MOHAMMAD

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Journal: Avicenna Journal of Clinical Medicine Year:2018 | Volume:25 | Issue:1 (SN 87) Page(s): 28-34

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Title

MOLECULAR DETECTION OF VIRULENCE GENES AMONG PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM CLINICAL SPECIMENS BY MULTIPLEX PCR

Author(s)

SHARIAT AFSOON | ALIZADEH SAJAD | JAHANGIRI HOSEINABADI MEHDI | MOVAGHARNEJAD MOHAMMAD | Issue Writer Certificate

Keywords

GENESQ1

MULTIPLEX PCRQ1

PSEUDOMONAS AERUGINOSAQ2

Abstract

Background and Objective: Pseudomonas aeroginosa is the most common pathogen associated with nosocomial infections and possesses virulence factors which contribute to the bacterial invasion and toxicity such as alginate, exoenzyme S, exotoxin A and elastase. The aim of this study was to determine the prevalence of algD, exoS, toxA and lasB GENES in PSEUDOMONAS AERUGINOSA samples isolated from patients by MULTIPLEX PCR.Materials and Methods: In this cross sectional study, sixty clinical samples were collected from Dey and Motahhari Hospitals, Tehran, Iran. Following identification of isolates by biochemical methods, Antibiogram test was performed using disc diffusion method with different antibiotics. MULTIPLEX PCR method was performed to identify the desired GENES.Results: 37 out of 60 (61.66%) male and 23 out of 60 (38.34%) female specimens were positive for PSEUDOMONAS AERUGINOSA. The highest level of antibiotic resistance of the Pseudomonas aeroginosa isolates was observed against ceftriaxone (93.33%). The prevalence rate of virulence GENES among all isolates was as follows; lasB (61.7%), toxA (60%), algD (43.3%) and exoS (5%).Conclusion: Elastase, exotoxin A and alginate are considered important virulence factors of PSEUDOMONAS AERUGINOSA and they play a major role in causing diseases and tissue and skin lesions.

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APA: Copy

SHARIAT, AFSOON, ALIZADEH, SAJAD, JAHANGIRI HOSEINABADI, MEHDI, & MOVAGHARNEJAD, MOHAMMAD. (2018). MOLECULAR DETECTION OF VIRULENCE GENES AMONG PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM CLINICAL SPECIMENS BY MULTIPLEX PCR. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), 25(1 (SN 87) ), 28-34. SID. https://sid.ir/paper/17921/en

Vancouver: Copy

SHARIAT AFSOON, ALIZADEH SAJAD, JAHANGIRI HOSEINABADI MEHDI, MOVAGHARNEJAD MOHAMMAD. MOLECULAR DETECTION OF VIRULENCE GENES AMONG PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM CLINICAL SPECIMENS BY MULTIPLEX PCR. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES)[Internet]. 2018;25(1 (SN 87) ):28-34. Available from: https://sid.ir/paper/17921/en

IEEE: Copy

AFSOON SHARIAT, SAJAD ALIZADEH, MEHDI JAHANGIRI HOSEINABADI, and MOHAMMAD MOVAGHARNEJAD, “MOLECULAR DETECTION OF VIRULENCE GENES AMONG PSEUDOMONAS AERUGINOSA STRAINS ISOLATED FROM CLINICAL SPECIMENS BY MULTIPLEX PCR,” AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), vol. 25, no. 1 (SN 87) , pp. 28–34, 2018, [Online]. Available: https://sid.ir/paper/17921/en

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