IMMUNOHISTOCHMICAL STUDY OF GLOMERULAR MESENGIAL COLLAGEN IV EXPRESSION IN DIABETIC BALB/C MICE

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JALALI M.; NIKRAVESH M.R.

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Journal: Avicenna Journal of Clinical Medicine Year:2006 | Volume:13 | Issue:1 (SN 39) Page(s): 5-11

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Title

IMMUNOHISTOCHMICAL STUDY OF GLOMERULAR MESENGIAL COLLAGEN IV EXPRESSION IN DIABETIC BALB/C MICE

Author(s)

JALALI M. | NIKRAVESH M.R. | Issue Writer Certificate

Keywords

COLLAGEN TYPE IVQ3

DIABETES MELLITUSQ3

GLOMERULAR BASEMENT MEMBRANEQ3

MICEQ3

Abstract

Introduction & Objective: Extra-cellular matrix and basement membrane play important roles in many developmental phenomenons during development and after birth. Among the components of the basement membrane, collagen fibers specially type IV, are the most important part of this area. As kidney is one of the target organs in DIABETES MELLITUS and diabetic nephropathy is a major cause of end stage-renal disease and result an increase in morbidity and mortality of effected individuals, therefore early diagnosis leads to better treatment. The aim of this investigation was to study the primary diagnostic parameters by special regards to collagen IV fibers.Materials & Methods: In this study, 24 male balb/c MICE were divided into experimental and control groups. In experimental group, the beta cells of Langerhance were chemically destroyed by an injection of 160 mg/kg alloxan and subdivided in experimental groups 1 and 2. Controls were kept untreated. Experimental group 1and 2 were sacrified 8 and 16 weeks after treatment with alloxan respectively. The same procedure was performed' for control group. Immunohistochmical studies were carried out using monoclonal atntibody. Against COLLAGEN TYPE IV in Glomeruli. In addition, using morphometrical and steredlogical methods the volume of the glumerles was compared in all groups. Results: Our finding showed that in experimental groups with special regards in 16 weeks diabetic MICE, the rate of COLLAGEN TYPE IV in basement membrane around the parietal layer of Bowman capsule, mesangial cells and endothelium of capillary in glomerules increased significantly compared to controls and experimented group 1 (p<0.05), while there was not a significant difference among experimental group 4 and controls. Our data also revealed that the number of mesangial cells as well as glomerular volume increased significantly in experimental 2 (4.635±0.289×106μm3) compared to experimental 1 (3.504±0.189×106μm3) and controls (3.422±0.140×106 μm3).Conclusion: This study indicated that any increase in the amount. of COLLAGEN TYPE IV in glomerules as well as mesangial cells and glomeruli volume which may happen; early in DIABETES MELLITUS, could be able to affect the physiology of the kidneys and it is a helpful tool for early diagnosis of affected kidney.

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APA: Copy

JALALI, M., & NIKRAVESH, M.R.. (2006). IMMUNOHISTOCHMICAL STUDY OF GLOMERULAR MESENGIAL COLLAGEN IV EXPRESSION IN DIABETIC BALB/C MICE. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), 13(1 (SN 39)), 5-11. SID. https://sid.ir/paper/18054/en

Vancouver: Copy

JALALI M., NIKRAVESH M.R.. IMMUNOHISTOCHMICAL STUDY OF GLOMERULAR MESENGIAL COLLAGEN IV EXPRESSION IN DIABETIC BALB/C MICE. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES)[Internet]. 2006;13(1 (SN 39)):5-11. Available from: https://sid.ir/paper/18054/en

IEEE: Copy

M. JALALI, and M.R. NIKRAVESH, “IMMUNOHISTOCHMICAL STUDY OF GLOMERULAR MESENGIAL COLLAGEN IV EXPRESSION IN DIABETIC BALB/C MICE,” AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), vol. 13, no. 1 (SN 39), pp. 5–11, 2006, [Online]. Available: https://sid.ir/paper/18054/en

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