SERUM-FREE CRYOPRESERVATION OF HUMAN AMNIOTIC EPITHELIAL CELLS

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NIKNEJAD H.; PEIROVI H.; JAMBAR NOUSHIN B.

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Journal Paper

Paper Information

Journal: Avicenna Journal of Clinical Medicine Year:2013 | Volume:20 | Issue:1 (SN 67) Page(s): 15-24

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Information Journal Paper

Title

SERUM-FREE CRYOPRESERVATION OF HUMAN AMNIOTIC EPITHELIAL CELLS

Author(s)

NIKNEJAD H. | PEIROVI H. | JAMBAR NOUSHIN B. | Issue Writer Certificate

Keywords

AMNIOTIC FLUIDQ2

CRYOPRESERVATIONQ2

EPITHELIAL CELLSQ2

GLYCEROLQ2

Abstract

Introduction & Objective: One of the important issues in long term storage of cells is removal of animal serum from cell culture environments. The aim of this study was to evaluate AMNIOTIC FLUID (AF), which is full of growth factors, as substitute for fetal bovine serum (FBS) in the CRYOPRESERVATION protocol.Materials & Methods: In this experimental study human amniotic EPITHELIAL CELLS were isolated from placentas which were seronegative for microbial infections. The cells were preserved in 24 different patterns for 12 months in -196oC (liquid nitrogen) and viability of cells were determined before and after CRYOPRESERVATION by trypan blue and MTT assay. Moreover, Oct-4 expression was studied to determine pluripotency before and after CRYOPRESERVATION with immunocytochemistry. Results were compared between groups with ANOVA (Tukey Post-Test). P.value under 0.01 and 0.05 was considered statistically significant.Results: The presence of DMEM, FBS or AF is necessary for amniotic cell CRYOPRESERVATION.Trypan-blue, MTT and immunocytochemistry showed that there isn’t significant difference between using AF and FBS in viability and pluripotency of cells. Moreover, results showed that DMSO is a better cryoprotectant compared to GLYCEROL.Conclusion: Results showed that AMNIOTIC FLUID can be a proper substitute for FBS in amniotic EPITHELIAL CELLS CRYOPRESERVATION.

Cites

EFFECT OF ETHYL ACETATE EXTRACT OF SEA PEN VIRGULARIA GUSTAVIANA ON VIABILITY OF CANCER CELLS

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APA: Copy

NIKNEJAD, H., PEIROVI, H., & JAMBAR NOUSHIN, B.. (2013). SERUM-FREE CRYOPRESERVATION OF HUMAN AMNIOTIC EPITHELIAL CELLS. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), 20(1 (SN 67)), 15-24. SID. https://sid.ir/paper/18085/en

Vancouver: Copy

NIKNEJAD H., PEIROVI H., JAMBAR NOUSHIN B.. SERUM-FREE CRYOPRESERVATION OF HUMAN AMNIOTIC EPITHELIAL CELLS. AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES)[Internet]. 2013;20(1 (SN 67)):15-24. Available from: https://sid.ir/paper/18085/en

IEEE: Copy

H. NIKNEJAD, H. PEIROVI, and B. JAMBAR NOUSHIN, “SERUM-FREE CRYOPRESERVATION OF HUMAN AMNIOTIC EPITHELIAL CELLS,” AVICENNA JOURNAL OF CLINICAL MEDICINE (SCIENTIFIC JOURNAL OF HAMADAN UNIVERSITY OF MEDICAL SCIENCES AND HEALTH SERVICES), vol. 20, no. 1 (SN 67), pp. 15–24, 2013, [Online]. Available: https://sid.ir/paper/18085/en

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