IDENTIFICATION OF MOLECULAR PATTERN OF INDOOR & OUTDOOR OF 2 SPECIES ASPERGILLUS (FUMIGATUS & FLAVUS) IN THE HOSPITAL

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HOSSEINI N.; BAYAT MANSOUR; ROUDBAR MOHAMMADI S.

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Journal: JOURNAL OF MICROBIAL BIOTECHNOLOGY Year:2011 | Volume:3 | Issue:10 Page(s): 15-21

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Title

IDENTIFICATION OF MOLECULAR PATTERN OF INDOOR & OUTDOOR OF 2 SPECIES ASPERGILLUS (FUMIGATUS & FLAVUS) IN THE HOSPITAL

Author(s)

HOSSEINI N. | BAYAT MANSOUR | ROUDBAR MOHAMMADI S. | Issue Writer Certificate

Keywords

MOLECULAR PATTERNQ2

ASPERGILLUS FUMIGATUSQ2

ASPERGILLUS FLAVUSQ2

NOSOCOMIAL INFECTIONSQ2

RAPD-PCRQ2

Abstract

Background and Objective: Aspergillus spp can cause various diseases including: invasive aspergillosis (IA), chronic necrotizing aspergillosis, aspergilloma and allergic bronchopulmonary aspergillosi. Most case of IA caused by Aspergillus fumigatues. ASPERGILLUS FLAVUS being often reported as the second most frequent species. NOSOCOMIAL INFECTIONS occur worldwide. They are important contributors to morbidity and mortality and ASPERGILLUS FUMIGATUS play an important role in these infections. Clinical microbiology laboratories in tertiary-care hospitals should be able to perform epidemiological analysis and surveillance of aspergilla in a hospital environment on routine basis.Materials and Methods: We collected samples of air from outdoor and indoor environment in the hospital in summer, autumn, winter and spring. Czapeck agar plates were used as culture medium. Among the methods suitable for identification RAPD offers the best potential in terms of obtaining discriminatory data in a rapid simple and cost-efficient way.Results: The autumn collection yielded more isolates than that of the other seasons. Several RAPD primers and protocols have been published for using in different species of the genus Aspergillus. Among the primers tested R108 show an unequivocal differentiation of ASPERGILLUS FUMIGATUS and ASPERGILLUS FLAVUS.Conclusion: A reliable simple and rapid molecular method with high discriminatory power is necessary for identifying the MOLECULAR PATTERN of each species.

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APA: Copy

HOSSEINI, N., BAYAT, MANSOUR, & ROUDBAR MOHAMMADI, S.. (2011). IDENTIFICATION OF MOLECULAR PATTERN OF INDOOR & OUTDOOR OF 2 SPECIES ASPERGILLUS (FUMIGATUS & FLAVUS) IN THE HOSPITAL. JOURNAL OF MICROBIAL BIOTECHNOLOGY, 3(10), 15-21. SID. https://sid.ir/paper/181415/en

Vancouver: Copy

HOSSEINI N., BAYAT MANSOUR, ROUDBAR MOHAMMADI S.. IDENTIFICATION OF MOLECULAR PATTERN OF INDOOR & OUTDOOR OF 2 SPECIES ASPERGILLUS (FUMIGATUS & FLAVUS) IN THE HOSPITAL. JOURNAL OF MICROBIAL BIOTECHNOLOGY[Internet]. 2011;3(10):15-21. Available from: https://sid.ir/paper/181415/en

IEEE: Copy

N. HOSSEINI, MANSOUR BAYAT, and S. ROUDBAR MOHAMMADI, “IDENTIFICATION OF MOLECULAR PATTERN OF INDOOR & OUTDOOR OF 2 SPECIES ASPERGILLUS (FUMIGATUS & FLAVUS) IN THE HOSPITAL,” JOURNAL OF MICROBIAL BIOTECHNOLOGY, vol. 3, no. 10, pp. 15–21, 2011, [Online]. Available: https://sid.ir/paper/181415/en

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